A novel method for isolation of Campylobacter spp. from environmental samples, involving sample processing, and blood- and antibiotic-free medium

Journal of Applied Microbiology
M BaserisalehiB P Kapadnis

Abstract

To develop a method that involves sample processing, and blood- and antibiotic-free medium for isolation and enumeration of Campylobacter spp. from environmental samples. The sample processing (preT) was standardized to minimize the population of competing bacteria. A blood- and antibiotic-free differential, Kapadnis-Baseri medium (KB medium) was formulated and tested for isolation of Campylobacter spp. in comparison with CAT medium. PreT-KB method was evaluated in comparison with the conventional viable count method and with the conventional most probable number (C. MPN) method for enumeration of Campylobcater from environmental samples. The results indicated that sample processing significantly reduced population of competing bacteria. The KB medium selected Gram-negative bacteria and differentiated Campylobacter from lactose-fermenting competing bacteria. The population of Campylobacter detected by preT-KB method was similar to that by conventional viable count method. While, the population of Campylobacter spp. determined by preT-KB method was higher than that by C. MPN method. In addition, the preT-KB method detected antibiotic sensitive campylobacters. The preT minimizes population of competing bacteria and the KB medium ...Continue Reading

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Citations

Nov 13, 2013·International Journal of Environmental Research and Public Health·Harriet WhileyRichard Bentham
May 1, 2007·Pakistan Journal of Biological Sciences : PJBS·M BaserisalehiB P Kapadnis
Aug 4, 2009·Journal of Food Science·R OdongoS E Gilliland
Jul 13, 2006·Letters in Applied Microbiology·M R KhannaB P Kapadnis
May 24, 2006·Microbes and Infection·John E MooreOla Tolba

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