A novel method for isolation of Chlamydia pneumoniae by treatment with trypsin or EDTA.

Journal of Clinical Microbiology
Y KazuyamaF Taguchi

Abstract

To establish a novel method for the efficient isolation of Chlamydia pneumoniae, experiments were performed to determine the effects of EDTA or trypsin treatment of C. pneumoniae on its adsorption and inclusion body formation. Treatment of C. pneumoniae with 0.1% trypsin or 1 mM EDTA significantly increased inclusion body-forming activity from 8,000- to 10,000-fold higher than that of the control. C. pneumoniae was successfully isolated in cultured cells which were inoculated with clinical specimens after treatment with 0.1% trypsin.

References

Jul 1, 1992·Nihon saikingaku zasshi. Japanese journal of bacteriology·N KidaF Taguchi
Feb 1, 1992·Journal of Clinical Microbiology·L A CampbellJ T Grayston
Sep 1, 1990·The Journal of Infectious Diseases·C C Kuo, J T Grayston
Mar 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J T GraystonL A Campbell
Feb 1, 1988·The Journal of Infectious Diseases·M KleemolaJ T Grayston
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Jun 1, 1972·The Journal of Infectious Diseases·C KuoJ T Grayston
Sep 1, 1994·Nihon saikingaku zasshi. Japanese journal of bacteriology·N KidaF Taguchi

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Citations

May 4, 1999·Scandinavian Journal of Infectious Diseases·T MachiY Horita
Jul 20, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S F DowellUNKNOWN C. pneumoniae Workshop Participants
Sep 17, 1999·Journal of Clinical Microbiology·J M PrucklerB S Fields
Jul 3, 1998·Journal of Clinical Microbiology·C A JantosJ H Hegemann

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