PMID: 10357200Jun 5, 1999Paper

A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry

Journal of Immunological Methods
J E GoldbergC Clayberger

Abstract

An assay based on two-color flow cytometry has been developed to measure CTL and NK cell-mediated cytotoxicity. After effector/target cells are incubated together, CTL or NK populations are stained with an effector cell specific PE-conjugated mAb. Subsequently, annexin V-FITC binds to cells expressing phosphatidylserine (an early marker of apoptosis) on the cell surface. Target cells are gated upon as PE-negative and quantified with respect to their annexin V positivity. The shift from annexin Vneg to annexin Vhi is a discrete event such that all target cells fall within discernible populations with respect to annexin V. There is a strong correlation between cytotoxicity measured with our assay and a standard 51Cr release assay (r2 = 0.989). The PE/annexin V assay shows increased sensitivity at early timepoints after target/effector cell mixing. In addition, this method allows for analysis of target cells at the single cell level. Therefore, we have described a promising new technique to measure in vitro cell-mediated cytotoxicity. It avoids the potential difficulties of working with radioactive isotopes, and offers increased sensitivity and versatility.

References

Feb 14, 1992·Journal of Immunological Methods·H OhmoriY Honma
Dec 15, 1991·Journal of Immunological Methods·A SuhrbierD J Moss
Aug 15, 1989·Journal of Immunological Methods·W G WierdaY B Kim
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A M KrenskyS J Burakoff
Dec 28, 1994·Journal of Immunological Methods·N G PapadopoulosM Papamichail
Dec 28, 1994·Journal of Immunological Methods·L KarawajewK Ganzel
Nov 5, 1993·Journal of Immunological Methods·L ChangS L Nehlsen-Cannarella
Mar 15, 1993·Journal of Immunological Methods·R F HussainR T Oliver
May 26, 1997·Journal of Immunological Methods·A E MattisR Förster

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Citations

Feb 26, 2005·Experimental Hematology·Hitomi KanekoTakashi Uchiyama
Feb 28, 2001·Journal of Immunological Methods·M E SheehyD F Nixon
Sep 7, 2002·Journal of Immunological Methods·Yan-Yi Wang, Xiao-Xiang Zheng
Dec 31, 2002·Journal of Immunological Methods·Wannee KantakamalakulKovit Pattanapanyasat
Sep 13, 2003·Veterinary Immunology and Immunopathology·Yongqiang WangOlli Vainio
Feb 13, 2003·Toxicology·Karin CederbrantJacques Descotes
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Paul J Choi, Timothy J Mitchison
Oct 26, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Faribourz PayvandiDavid I Stirling
Aug 23, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Greg M LandryKenneth E McMartin
Oct 27, 2004·Journal of Translational Medicine·Nigel J WaterhouseChris Jp Clarke
Apr 7, 2011·International Journal of Nanomedicine·Nagehan SaltanRidvan Say
May 5, 2011·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Mar 2, 2010·Developmental and Comparative Immunology·Christine A JansenLonneke Vervelde
Aug 18, 2009·Journal of Immunological Methods·Pranav GuptaSubrat Kumar Panda
Mar 4, 2008·Methods : a Companion to Methods in Enzymology·Vivien R SuttonJoseph A Trapani
May 17, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Theresia M WestersArjan A van de Loosdrecht
Feb 17, 2006·European Journal of Haematology·Feng ZhangMing Hou
Apr 28, 2006·International Journal of Cancer. Journal International Du Cancer·Martin KornackerAnthony D Ho
Oct 18, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Karina Godoy-RamirezHans Gaines
Jun 29, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Benjamin J C QuahChristopher R Parish
Apr 18, 2003·AIDS·Christian Brander, Yves Rivière
Jun 4, 2010·Expert Review of Vaccines·Liubov ZaritskayaAnatoli M Malyguine
Oct 28, 2008·Immunity·Sunil S MetkarChristopher J Froelich
Feb 7, 2001·Immunity·G Hernández-HoyosJ Alberola-Ila
Jan 13, 2015·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Jeremiah L OyerAlicja J Copik
Dec 26, 2016·The Journal of Investigative Dermatology·Gang LiPatrizia Fuschiotti

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