PMID: 8598279Jan 1, 1996Paper

A novel method for the isolation of mycobacterial DNA

FEMS Microbiology Letters
J A Gonzalez-y-MerchandR A Cox

Abstract

DNA was isolated from mycobacteria by a simplified procedure. Cells were suspended in 6 M guanidinium chloride, the suspension was cooled to -70 degrees C, then incubated at 65 degrees C for 10 min, cooled in ice, deproteinized by chloroform and DNA was recovered from the supernatant. The procedure was used to obtain DNA from several mycobacteria (1 x 10(9) or more cells) including Mycobacterium neoaurum, M. fortuitum, M. phlei and M. smegmatis. Each of the species was shown to have two ribosomal RNA operons per genome, and preliminary evidence was obtained which suggests that one of these operons is homologous with one of the operons of M. smegmatis.

References

May 30, 1975·Molecular and Cellular Biochemistry·E LedererJ Wietzerbin
Jul 1, 1992·International Journal of Systematic Bacteriology·C PitulleE Stackebrandt
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Oct 1, 1990·International Journal of Systematic Bacteriology·T RogallE C Böttger
May 14, 1986·Biochemical and Biophysical Research Communications·H BercovierS Sela
Apr 1, 1986·Nihon juigaku zasshi. The Japanese journal of veterinary science·S TakaiS Tsubaki
Nov 1, 1994·Analytical Biochemistry·T ImaiJ Tobari

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Citations

Jan 18, 2007·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Xin JiangHonghai Wang
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Rosilene Fressatti CardosoMario H Hirata
Dec 3, 2008·Applied and Environmental Microbiology·Michael KäserGerd Pluschke
Oct 22, 2003·FEMS Microbiology Letters·Sandra Rivera-GutiérrezJorge A Gonzalez-y-Merchand
Oct 15, 2010·Memórias do Instituto Oswaldo Cruz·Erika Noda NogutiRosilene Fressatti Cardoso
Feb 13, 2007·Memórias do Instituto Oswaldo Cruz·Rosilene Fressatti CardosoMario Hiroyuki Hirata
Nov 26, 1997·Journal of Bacteriology·J A Gonzalez-y-MerchandR A Cox
Apr 3, 2010·Cold Spring Harbor Protocols·Michael KäserGerd Pluschke

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