A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling

Experientia
A Castro, N Monji

Abstract

Meta-maleimidobenzoyl derivative of L-thyroxine methyl ester (MBTM) was synthesized and coupled to beta-galactosidase at molar ratio of over 5 to 1. More than 97% of the enzyme was found to be labeled with MBTM. A thyroxine enzyme immunoassay was carried out with sensitivity in 0-10 microgram/100 ml range.

References

Mar 1, 1976·Journal of Immunological Methods·S Comoglio, F Celada
Jan 1, 1976·Journal of Biochemistry·T Kitagawa, T Aikawa
Sep 1, 1971·The Journal of Clinical Endocrinology and Metabolism·H GharibT Hockert
Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
Jan 1, 1928·The Biochemical Journal·J N Ashley, C R Harington

Citations

Aug 10, 2000·Journal of Immunoassay·J E Butler
Jan 1, 1980·Journal of Immunoassay·A H Schuurs, B K van Weemen

Related Concepts

Metazoa
Lactrase
Goat
Haptens
Peroxidase-Antiperoxidase Complex Technique
Maleic Anhydride
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Levoxyl

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