A novel method of labeling and characterizing migrating neurons in the developing central nervous system

Experimental Neurology
Pavlos AlifragisBagirathy Nadarajah

Abstract

Neuronal migration, a discrete event in the developing nervous system, is currently being intensively investigated using a variety of anatomical and molecular approaches. Using 4-chloromethyl benzoyl amino tetramethyl rhodamine (CMTMR) coated particles, we describe here a novel and efficient method of tracer labeling to investigate cell migration in embryonic and postnatal brain. Further, we demonstrate that application of CMTMR facilitates the labeling of a large number of migrating cells and enables the characterization of their phenotypes with immunohistochemical and in situ hybridization techniques. We also illustrate that CMTMR labeling is ideally suited for time-lapse imaging of the behavior and dynamics of migrating cells.

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Citations

Feb 12, 2010·Cold Spring Harbor Protocols·Wen-Biao GanJeff W Lichtman
Dec 1, 2003·Integrative and Comparative Biology·Stuart A TobetKwok W Yu
Mar 15, 2003·Progress in Neurobiology·Christine Y BrazelSteven W Levison
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Aug 12, 2006·Developmental Biology·Olga BritanovaVictor Tarabykin
Aug 18, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·L K FriedmanH Hong
Dec 14, 2007·Developmental Neuroscience·Anastasia LiapiBagirathy Nadarajah
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Lara López-GonzálezLuis Puelles

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