A novel method of monitoring response to islet transplantation: bioluminescent imaging of an NF-kB transgenic mouse model

Transplantation
David J RothTaylor G Wang

Abstract

Transplantation of encapsulated pancreatic islets is a novel therapeutic approach for the treatment of Type 1 diabetes mellitus that has the potential to circumvent both a limited islet supply and immunosuppression. Current methods for scoring the biocompatibility of the alginate-based capsules that sequester Islets of Langerhans include fabrication and implantation into the peritoneal cavity of mice, incubation, retrieval via peritoneal lavage, and observation of the number of cells or cell layers surrounding the capsules. This method allows only one data point to be obtained per animal. We describe a method to measure biocompatibility real time and in situ. This method of monitoring immune response using bioluminescent technology and a nuclear factor-kappa beta (NF-kB) sensitive transgenic mouse model allows many data points to be acquired per animal, reduces the number of animals required to obtain statistically significant immune response data over time, and in turn reduces error associated with animal variability. NF-kB is a transcription factor that coordinates the inflammatory and wound healing cascades by initiating the transcription of cytokines, chemokines, adhesion molecules, and proinflammatory genes. Inflammation a...Continue Reading

References

Oct 15, 1996·Transplantation·P De VosR Van Schilfgaarde
Jul 1, 1997·American Journal of Respiratory Cell and Molecular Biology·T S Blackwell, J W Christman
Jan 16, 1998·Journal of Biomedical Materials Research·I LacíkT Wang
May 16, 2000·Chest·J W ChristmanT S Blackwell
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·T S BlackwellL D Kerr
Sep 11, 2001·American Journal of Respiratory and Critical Care Medicine·R T SadikotT S Blackwell
Dec 18, 2001·Biotechnology and Bioengineering·A V AnilkumarT G Wang
Apr 30, 2002·Inflammatory Bowel Diseases·David L BooneAveril Ma
May 7, 2002·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Ruxanna T SadikotWilliam C Chapman
Oct 31, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·N N Ugarova, L Y Brovko
Nov 16, 2002·Journal of Microencapsulation·B L StrandG Skjåk-Baek
Nov 16, 2002·Journal of Microencapsulation·S RosińskiD Hunkeler
Apr 1, 2001·Cell Transplantation·Berit L StrandTerje Espevik

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Citations

Sep 9, 2008·Diabetologia·D Holmberg, U Ahlgren
Aug 10, 2007·Current Diabetes Reports·Jason L Gaglia
Jan 4, 2008·ILAR Journal·Kurt R ZinnStuart J Frank
Oct 18, 2008·Diabetes, Obesity & Metabolism·Z Medarova, A Moore
Jan 1, 2009·American Journal of Physiology. Endocrinology and Metabolism·Patrick F AntkowiakRaghavendra G Mirmira
Oct 21, 2011·Journal of Transplantation·Ping WangAnna Moore
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Mai LinXiankai Sun
Sep 14, 2011·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Vanessa M Dimayuga, Martin Rodriguez-Porcel
Sep 22, 2010·Advanced Drug Delivery Reviews·Zhanhong Wu, Fouad Kandeel
Jul 14, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·You-Cheng HseuChien-Yun Hsiang
Nov 21, 2007·Advanced Drug Delivery Reviews·John T Wilson, Elliot L Chaikof
Jul 31, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Lian-Li MaDeng-Ping Yin
Aug 18, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M L MalosioA Del Maschio
Jul 21, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Q GaoD P Yin
Feb 15, 2015·The International Journal of Biochemistry & Cell Biology·Snehal M GaikwadPritha Ray
Oct 11, 2016·World Journal of Radiology·Junfeng LiFouad Kandeel
Jun 24, 2009·Nature Reviews. Endocrinology·Zdravka Medarova, Anna Moore
Apr 2, 2019·Frontiers in Pharmacology·Isabella ManniGiulia Piaggio
Nov 5, 2020·International Journal of Molecular Sciences·Tzu-Yin ChenKurt Ming-Chao Lin
Dec 14, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Joo Ho TaiSavita Dhanvantari

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