PMID: 3759665Aug 1, 1986Paper

A novel method of screening for immunomodulating substances, establishment of an assay system and its application to culture broths of microorganisms

The Journal of Antibiotics
A NakamuraG Tamura

Abstract

A novel method of screening for immunomodulating substances is developed employing lymphocytes and three mitogens. Concanavalin A (Con A) and phytohemagglutinin (PHA) are applied as T cell specific stimulants and lipopolysaccharide (LPS) as a B cell specific stimulant respectively. The lymphocytes obtained from mouse spleen are cultured with an antibiotic or a sample extract in the presence or absence of mitogen for three days and pulsed with [3H]thymidine for five hours before harvest. Differential effects of a sample compound on [3H]thymidine incorporation by the activated and quiescent lymphocytes are scored. In this procedure most of the tested antibiotics or chemical compounds with different mode of actions show non-specific effects. Cyclosporin A, a potent immunosuppressive substance, suppresses both Con A and PHA responses more extensively than LPS response and quiescent cell growth, and two cytochrome bc1 complex inhibitors, funiculosin and antimycin A3, are less suppressive to PHA response than to the others. The present system was also applied to the methanol extracts of the culture broths prepared from the type strains of Actinomycetes and Penicillium.

Citations

Jan 1, 1991·Folia Microbiologica·Z VanĕkE Curdová
Nov 29, 2007·Future Microbiology·Neil R WilliamsonGeorge P C Salmond
Dec 23, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nouha Nasr-BouzaieneKamel Ghedira
Oct 16, 2007·Journal of Nutritional Science and Vitaminology·Hajime OtaniTakayoshi Aoki
Aug 9, 2008·Phytotherapy Research : PTR·Aurasorn SaraphanchotiwitthayaPattana Sripalakit
Dec 23, 2017·Journal of Natural Medicines·Haruhiro Fujimoto
Jul 21, 2011·Journal of Immunotoxicology·Mohamed Ben SghaierLeila Chekir-Ghedira
Mar 25, 2011·Immunopharmacology and Immunotoxicology·Mohamed Ben SghaierLeila Chekir-Ghedira
Jan 1, 1991·Critical Reviews in Biotechnology·C M Franco, L E Coutinho

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