A novel method to quantify in vivo transferrin glycation: applications in diabetes mellitus

Clinica Chimica Acta; International Journal of Clinical Chemistry
Ann Van CampenhoutBegoña Manuel-y-Keenoy

Abstract

In vitro glycation of transferrin leads to increased oxidative stress by impairing iron-binding antioxidant capacity. The aim of this study is to develop a method to evaluate in vivo transferrin glycation in diabetes. We adapted the nitroblue tetrazolium assay to measure in micro-well plates the fructosamine content of transferrin isolated from serum by immunocomplexation. Introduction of the immunocomplexation step did not affect the analytical performance of the fructosamine measurement and analytical variability was lower than 7%. The diabetic group (n=107) had significantly higher transferrin glycation (1.39+/-1.12 versus 0.79+/-1.09 micromol fructosamine/g transferrin in the non-diabetic group, n=91, p<0.0005) and this was most pronounced in type 1 diabetes (1.95+/-1.02 versus 1.06+/-1.04 micromol fructosamine/g transferrin in type 2, p<0.0005). Transferrin glycation was associated with parameters of glycaemic control but did not correlate with serum iron or total iron-binding capacity. Total iron-binding capacity was lower in type 1 diabetes (63+/-9 versus 69+/-12 micromol/l in type 2, p<0.05) and was mainly determined by transferrin concentration. These results indicate that the adapted nitroblue tetrazolium assay combin...Continue Reading

References

Apr 1, 1979·FEBS Letters·R Dolhofer, O H Wieland
Aug 1, 1991·Diabetic Medicine : a Journal of the British Diabetic Association·M J O'DonnellA H Barnett
Dec 31, 1990·Biochemical and Biophysical Research Communications·C J MullarkeyM Brownlee
Feb 13, 1986·The New England Journal of Medicine·V M MonnierR R Kohn
Sep 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B J PotterS Sherlock
Sep 1, 1984·The Journal of Pediatrics·S F KempT R Horn
Jan 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·R N JohnsonJ R Baker
May 1, 1995·The American Journal of Medicine·A GriesmacherG Schernthaner
Feb 17, 1996·Lancet·P DandonaT Nicotera
Feb 19, 1999·Metabolism: Clinical and Experimental·M InouyeK Sumino
Dec 10, 1999·Diabetes Care·E S Ford, M E Cogswell
Mar 22, 2000·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A TelciT Akçay
Dec 28, 2002·Diabetes Care·UNKNOWN American Diabetes Association
Jan 1, 1961·Scandinavian Journal of Clinical and Laboratory Investigation·S JARNUM, N A LASSEN
Sep 23, 2003·Biophysical Chemistry·Brian L CussimanioRaja G Khalifah
Jan 6, 2004·Free Radical Research·Ann van CampenhoutBegoña Manuel-y-Keenoy
Apr 24, 2004·Free Radical Research·Anjana RoyAbhay Sankar Chakraborti
Jun 23, 2004·Journal of Diabetes and Its Complications·Ramazan Memişoğullari, Ebubekir Bakan
Jul 3, 2004·Clinical Chemistry·Ann Van CampenhoutBegoña Manuel-Y-Keenoy
Dec 29, 2004·Biophysical Chemistry·Subhrojit SenAbhay Sankar Chakraborti

❮ Previous
Next ❯

Citations

May 22, 2013·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Anders Helander, Naama Kenan Modén
Jun 3, 2014·BMC Gastroenterology·Kevin J FaganElizabeth E Powell
Jun 23, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ann Van CampenhoutBegoña Manuel-y-Keenoy
Aug 27, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Monique van ScherpenzeelDirk J Lefeber
May 9, 2006·Free Radical Biology & Medicine·Ann Van CampenhoutBegoña Manuel-y-Keenoy
Jun 18, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Yanqi MaSongbo Fu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved