A novel microencapsulated peptide vaccine against hepatitis B

Vaccine
J S MoynihanC R Howard

Abstract

A 48 amino acid synthetic peptide (S121/48) representing residues 121-167 of the major envelope protein of hepatitis B virus (HBsAg) was successfully encapsulated into polylactide co-glycolide microspheres. A single immunization of the microspheres in BALB/c (H-2d) mice resulted in the production of high-titre anti-HBs antibodies (IgG1-type). The response was long lasting and was superior to that obtained using the same peptide adjuvanted with Freund's complete adjuvant. A T-cell memory response was detected 10 weeks after a booster immunization (approximately 35 weeks after initial immunization) as measured by in-vitro re-stimulation of splenocytes. This study illustrates the feasibility of a single dose vaccine for hepatitis B and is, to our knowledge, the first demonstration of a synthetic peptide immunogen inducing anti-native protein antibodies of comparable titre to those obtained with conventional vaccines for hepatitis B. The suitability of a synthetic peptide vaccine for hepatitis B is discussed.

References

Mar 1, 1991·Molecular Immunology·J H EldridgeR M Gilley
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Rajadhyaksha, Y Thanavala
Jan 1, 1995·Journal of Viral Hepatitis·P Karayiannis, M J McGarvey
Aug 7, 1997·Journal of Immunological Methods·C D PartidosM W Steward
Aug 1, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·H TakahataS S Davis

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Citations

Jan 16, 2010·The AAPS Journal·Dilip PawarSuresh P Vyas
Feb 26, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Arvind K JainSuresh P Vyas
Dec 8, 2010·Vaccine·Rebecca S TirabassiKejian Yang
Jun 14, 2016·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Azmat Ali KhanAbdul Arif Khan

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