A novel microfluidic platform for studying mammalian cell chemotaxis in different oxygen environments under zero-flow conditions

Biomicrofluidics
Wei YangQi Ouyang

Abstract

The cell's micro-environment plays an important role in various physiological and pathological phenomena. To better investigate in vivo cellular behaviors, researchers have expended great effort in building controlled in vitro biophysical and biochemical environments. Because a cell's gaseous environment affects properties such as its division, metastasis, and differentiation, we developed a zero-flow based platform for studying mammalian cell chemotaxis behavior in different oxygen environments. This platform can construct a linear range of oxygen tensions within one chip (i.e., from 1.4% to 3.6% or 5.5% to 14.5%). To study cell chemotaxis behavior under varying oxygen environments, the chemical gradient direction is established perpendicularly to oxygen change within an observation area. Because the observation area is not subject to flow, shear force is of no concern. In addition, water flow around the cell chambers greatly reduces evaporation and makes long-term microscope imaging possible. In this study, we precisely measure the chemotaxis velocity of MCF-7 human breast cancer cells under different oxygen tension conditions towards CXCL12, which is a stromal cell-derived factor. We find that cell migration rates are not eq...Continue Reading

References

Dec 1, 1993·Enzyme and Microbial Technology·L Lebrun, G A Junter
Nov 15, 1997·Cancer·E C WoodhouseL A Liotta
Apr 27, 2001·Seminars in Cancer Biology·J S CondeelisJ E Segall
Sep 26, 2003·The American Journal of Pathology·Tsutomu ImaiIkuo Konishi
Nov 5, 2003·The Journal of Experimental Medicine·Tiziana SchioppaAntonio Sica
Jul 16, 2004·Seminars in Radiation Oncology·Peter Vaupel
Jul 28, 2006·Nature·George M Whitesides
Dec 14, 2006·Cancer Metastasis Reviews·Jianhua WangRussell S Taichman
Dec 21, 2006·Lab on a Chip·Thomas M Pearce, Justin C Williams
Apr 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Naz Chaudary, Richard P Hill
Apr 13, 2007·Nature·Gerhard Christofori
Apr 27, 2007·Cancer Metastasis Reviews·Richard Sullivan, Charles H Graham
Jun 26, 2007·FEBS Letters·M Christiane Brahimi-Horn, Jacques Pouysségur
Dec 12, 2007·Cancer Metastasis Reviews·Karen M BussardAndrea M Mastro
Aug 30, 2008·Science·Katrina PodsypaninaHarold Varmus
Nov 7, 2008·Nature Reviews. Cancer·Jessica A BertoutM Celeste Simon
Apr 18, 2009·Molecular Cancer Research : MCR·Amy M McCordPhilip J Tofilon
Feb 25, 2010·Chemical Society Reviews·Edmond W K Young, David J Beebe
Jun 18, 2010·Lab on a Chip·Kshitiz GuptaAndre Levchenko
Aug 5, 2010·Cell Stem Cell·Ahmed MohyeldinAlfredo Quiñones-Hinojosa
Oct 20, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Sudong KimNoo Li Jeon
Mar 26, 2011·Science·Christine L Chaffer, Robert A Weinberg
Jul 23, 2011·Nature Reviews. Cancer·Evanthia T RoussosAntonia Patsialou
Oct 18, 2011·Cell·Scott Valastyan, Robert A Weinberg
Sep 27, 2012·Lab on a Chip·Micha AdlerAlex Groisman
Jun 21, 2013·Biomedical Microdevices·Wei YangYugang Wang
Sep 25, 2014·Lab on a Chip·Martin D BrennanDavid T Eddington

❮ Previous
Next ❯

Citations

May 11, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kenichi FunamotoRoger D Kamm
Nov 15, 2018·Micromachines·Cheng LiAoqun Jian
Oct 21, 2020·Translational Oncology·Jia-Shun WuXin-Hua Liang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.