A novel mode of action of an ArfGAP, AMAP2/PAG3/Papa lpha, in Arf6 function

The Journal of Biological Chemistry
Shigeru HashimotoHisataka Sabe

Abstract

Previously we reported that AMAP2/PAG3/Papalpha/KIAA0400, a GTPase-activating protein (GAP), acts to antagonize Arf6 function when overexpressed, whereas it was shown to exhibit efficient GAP activities for other Arf isoforms in vitro. Here, we found that AMAP2, through its ArfGAP domain, binds to GTP-Arf6 but not to GDP-Arf6 or other Arfs irrespective of nucleotide status. The majority of AMAP2 was localized to intracellular tubulovesicular structures and redistributed to Arf6-enriched membrane areas upon Arf6 activation. In HeLa cells, Arf6 has been shown to be involved in the clathrin-independent endocytosis of Tac, but not the clathrin-dependent endocytosis of transferrin. We found that Arf6 silencing inhibited the internalization of Tac, but not transferrin, in HeLa cells. Internalization of Tac, but not transferrin, was also significantly inhibited by AMAP2 silencing and overexpression. AMAP2 was moreover found to bind to amphiphysin IIm, a component of the endocytic machinery, via its proline-rich domain. We propose that AMAP2 has dual mechanisms for its function; it exhibits efficient catalytic GAP activity for the class I and II Arfs and yet is involved in the cellular function of the class III Arf without immediate GA...Continue Reading

References

Feb 24, 1995·Science·C D'Souza-SchoreyP D Stahl
Mar 15, 1996·Science·R Schekman, L Orci
Aug 1, 1996·The Journal of Cell Biology·H RadhakrishnaJ G Donaldson
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·R UrrutiaM A McNiven
Aug 1, 1997·Current Biology : CB·P WiggeH T McMahon
Oct 6, 1997·The Journal of Cell Biology·H Radhakrishna, J G Donaldson
Mar 14, 1998·The Journal of Cell Biology·C D'Souza-SchoreyP J Peters
Aug 1, 1998·The Journal of Biological Chemistry·Q ZhangS Greenberg
Aug 11, 1998·Trends in Biochemical Sciences·K ScheffzekA Wittinghofer
Aug 15, 1998·The Journal of Biological Chemistry·J Moss, M Vaughan
Feb 18, 1999·Molecular and Cellular Biology·J AndreevJ Schlessinger
Apr 29, 1999·Cell·S SpringerR Schekman
Dec 22, 1999·The Journal of Experimental Medicine·E S GoldA Aderem
Apr 25, 2000·Molecular and Cellular Biology·R L BoshansC D'Souza-Schorey
Feb 24, 2001·Methods in Enzymology·J G Donaldson, H Radhakrishna
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·M Becker-HapakS F Dowdy
Sep 6, 2001·The Journal of Cell Biology·F D BrownJ G Donaldson
Sep 25, 2001·Trends in Cell Biology·B J Nichols, J Lippincott-Schwartz
Oct 5, 2001·Nature Cell Biology·N K HussainP S McPherson
Dec 12, 2001·Trends in Biochemical Sciences·K D Corbett, T Alber
Dec 10, 2002·Annual Review of Physiology·Helen L Yin, Paul A Janmey

❮ Previous
Next ❯

Citations

Dec 21, 2007·Molecular Biology of the Cell·Hajime YanoHisataka Sabe
Mar 4, 2011·Cell Structure and Function·Tomo FunakiMasanobu Satake
Jan 7, 2014·PloS One·Rucha KarnikAsipu Sivaprasadarao
Apr 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shigeru HashimotoHisataka Sabe
Oct 16, 2004·The EMBO Journal·Chie KojimaHisataka Sabe
Apr 6, 2016·The Journal of Cell Biology·Ari HashimotoHisataka Sabe
Mar 6, 2009·Cell Adhesion & Migration·Hisataka SabeEiji Ogawa
Mar 12, 2008·Experimental Cell Research·Shunsuke KonMasanobu Satake
Dec 14, 2004·The Journal of Biological Chemistry·Tanguy HoundoloAudrey Claing
Aug 2, 2007·Traffic·Hiroki Inoue, Paul A Randazzo
Apr 17, 2010·FEBS Letters·Anne SpangPaul A Randazzo
Mar 11, 2006·The Journal of Biological Chemistry·Stéphanie KleinFrédéric Luton
Feb 27, 2008·Seminars in Cell & Developmental Biology·Scott R Frank, Steen H Hansen
Mar 11, 2008·Trends in Cell Biology·Kenneth R Myers, James E Casanova

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.