A novel model of occlusive thrombus formation in mice

Laboratory Investigation; a Journal of Technical Methods and Pathology
Takeshi SasakiAkihisa Iguchi

Abstract

A novel model to induce occlusive thrombus formation was developed in mice in vivo. Mice were simultaneously treated with ligation and cuff placement at the left carotid artery. At 7 days after the treatment, occlusive thrombus was observed at the intracuff region, but not in the distal and proximal regions of the cuff, and not induced by a single treatment of ligation or cuff placement. The plasma levels of von Willebrand factor (vWF), which represent the endothelial status, were significantly increased in combined treatment of ligation and cuff placement 1 day after the operation. Whereas no significant changes in plasma vWF were observed in either single treatment of ligation or cuff placement. The expression of vWF, considered to be the endothelial marker, was detected on the luminal surface distal and proximal to the cuff and the carotid artery in the single treatment groups treated with either ligation or cuff placement, but was not detected in the intracuff region. Furthermore, the binding of Griffolia Simplicifolia Lectin-I (GSL-I) and endothelial nitric oxide synthase (eNOS) expression indicating the endothelial integrity was not detected in the intracuff region. Intermittent injections of ancrod, which decreases the p...Continue Reading

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Citations

Aug 2, 2012·Lipids in Health and Disease·Leandro C F LimaSilvana S Meyrelles
Sep 1, 2007·Journal of Cellular and Molecular Medicine·Ken A LindstedtPetri T Kovanen
Feb 1, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marina VilasecaJoan Carles García-Pagán
Apr 1, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Takeshi SasakiAkihisa Iguchi
Dec 23, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Erling FalkMichael E Rosenfeld
Jun 30, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Randal J WestrickDaniel T Eitzman
Sep 12, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Yasushi NumaguchiToyoaki Murohara
May 28, 2013·International Journal of Molecular Medicine·Chang Hoon HaKihwan Kwon

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