A novel monovalent cation channel activated by inositol trisphosphate in the plasma membrane of rat megakaryocytes.

The Journal of Biological Chemistry
B Somasundaram, M P Mahaut-Smith

Abstract

The activation of a monovalent cation current was studied in rat megakaryocytes using patch clamp techniques combined with photometric measurements of intracellular concentrations of Ca2+ ([Ca2+]i) and Na+. ADP evoked a release of [Ca2+]i and transiently activated a monovalent cation-selective channel, which, at negative potentials and under physiological conditions, would be expected to carry an inward Na+ current. The single channel conductance, estimated by noise analysis from whole cell currents at -50 to -60 mV was 9 picosiemens. Thapsigargin-induced [Ca2+]i increases failed to stimulate the monovalent cation current, suggesting that neither [Ca2+]i nor the depletion of internal Ca2+ stores were activators of this conductance. However, buffering of [Ca2+]i changes with 1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid showed that both activation and inactivation of the current were accelerated by a rise in [Ca2+]i. The monovalent cation conductance was activated by internal perfusion with inositol 1,4,5-trisphosphate, both in the presence and in the absence of a rise in [Ca2+]i. Internal perfusion with inositol 2,4,5-trisphosphate, the poorly metabolizable isomer of inositol trisphosphate, similarly activated the m...Continue Reading

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Citations

Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Tertyshnikova, A Fein
Jun 15, 1997·The Journal of Physiology·B SomasundaramM P Mahaut-Smith
Mar 2, 1999·The Journal of Physiology·M P Mahaut-SmithM J Mason
Jun 30, 2012·Journal of Thrombosis and Haemostasis : JTH·M P Mahaut-Smith
Jan 1, 2010·American Journal of Physiology. Cell Physiology·José P YoungAdam Myers
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Clara E MagyarPeter A Friedman
May 1, 1997·The American Journal of Physiology·E Romero, R Sullivan
May 1, 1997·Cell Calcium·S Tertyshnikova, A Fein

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