A novel motif in fungal class 1 histone deacetylases is essential for growth and development of Aspergillus.

Molecular Biology of the Cell
Martin TribusStefan Graessle

Abstract

Acetylation of the N-terminal tails of core histones is an important regulatory mechanism in eukaryotic organisms. In filamentous fungi, little is known about the enzymes that modify histone tails. However, it is increasingly evident that histone deacetylases and histone acetyltransferases are critical factors for the regulation of genes involved in fungal pathogenicity, stress response, and production of secondary metabolites such as antibiotics or fungal toxins. Here, we show that depletion of RpdA, an RPD3-type histone deacetylase of Aspergillus nidulans, leads to a pronounced reduction of growth and sporulation of the fungus. We demonstrate that a so far unnoticed motif in the C terminus of fungal RpdA histone deacetylases is required for the catalytic activity of the enzyme and consequently is essential for the viability of A. nidulans. Moreover, we provide evidence that this motif is also crucial for the survival of other, if not all, filamentous fungi, including pathogens such as Aspergillus fumigatus or Cochliobolus carbonum. Thus, the extended C terminus of RpdA-type enzymes represents a promising target for fungal-specific histone deacetylase-inhibitors that may have potential as novel antifungal compounds with medica...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Feb 1, 1989·Genetics·E B O'Hara, W E Timberlake
Jan 1, 1987·Gene·C E OakleyB R Oakley
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S E RundlettM Grunstein
Feb 1, 1997·Applied and Environmental Microbiology·S GraessleB Redl
Sep 30, 1998·Progress in Nucleic Acid Research and Molecular Biology·A P Wolffe, H Kurumizaka
Sep 29, 1999·Genes & Development·R E Kingston, G J Narlikar
Jan 19, 2000·Nature·B D Strahl, C D Allis
Feb 25, 2000·Molecular and Cellular Biology·M VignaliJ L Workman
Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B M Turner
Sep 27, 2000·Biochimica Et Biophysica Acta·S GraessleG Brosch
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·B E BernsteinS L Schreiber
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·D Shore
Dec 16, 2000·Trends in Biochemical Sciences·J Wu, M Grunstein
Jul 5, 2001·Cellular and Molecular Life Sciences : CMLS·S GraessleG Brosch
Sep 12, 2001·Progress in Nucleic Acid Research and Molecular Biology·B FelenbokI Nikolaev
Nov 10, 2001·Annual Review of Phytopathology·S L Tucker, N J Talbot
Feb 14, 2002·Chemistry & Biology·Christina M Grozinger, Stuart L Schreiber
Sep 5, 2002·Nature Reviews. Genetics·Lorna Casselton, Miriam Zolan
Nov 16, 2002·Science·Amy WangMichael Grunstein
Jul 8, 2003·Science of Aging Knowledge Environment : SAGE KE·Alejandro VaqueroDanny Reinberg
Jul 11, 2003·Nucleic Acids Research·Patrick TrojerStefan Graessle
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON
Nov 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexandra Lusser, James T Kadonaga
Feb 21, 2004·Methods in Enzymology·Daniel RobyrMichael Grunstein
Mar 31, 2004·Journal of Molecular Biology·Ivan V GregorettiHolly V Goodson
Apr 7, 2004·Cell·Philipp Korber, Wolfram Hörz
Jan 6, 2005·Molecular and Cellular Biology·Klavs R HansenGeneviève Thon
Aug 5, 2005·Annual Review of Phytopathology·Jae-Hyuk Yu, Nancy Keller
Nov 18, 2005·Gene·Michele A GlozakEdward Seto
Nov 30, 2005·Biochimica Et Biophysica Acta·Michael J CarrozzaJerry L Workman
Aug 17, 2006·Nature Reviews. Molecular Cell Biology·Catherine B Millar, Michael Grunstein

❮ Previous
Next ❯

Citations

Jul 18, 2012·Molecular and Cellular Biology·Motoyuki ShimizuNaoki Takaya
Oct 20, 2012·Fungal Genetics and Biology : FG & B·Md Kausar AlamSusan G W Kaminskyj
Mar 31, 2015·ACS Chemical Biology·Jessica C AlbrightNeil L Kelleher
Mar 13, 2015·Frontiers in Microbiology·Frédéric LamothWilliam J Steinbach
Nov 20, 2016·Applied and Environmental Microbiology·Macario Osorio-ConcepciónSergio Casas-Flores
Jun 4, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gnanendra ShanmugamJunhyun Jeon
Apr 16, 2019·Molecular Plant-microbe Interactions : MPMI·Huahui LanShihua Wang
Mar 15, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Natália Sayuri WassanoAndré Damasio
Mar 3, 2020·Frontiers in Microbiology·Ingo BauerStefan Graessle
Dec 24, 2019·Frontiers in Microbiology·Ingo BauerHubertus Haas
Nov 3, 2020·Frontiers in Microbiology·Junzhu ChenXiaotian Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
histone acetylation
PCR
Assay
electrophoresis
immunoprecipitation

Software Mentioned

COILS

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.