A novel mre11 mutation impairs processing of double-strand breaks of DNA during both mitosis and meiosis.

Molecular and Cellular Biology
H Tsubouchi, H Ogawa

Abstract

Using complementation tests and nucleotide sequencing, we showed that the rad58-4 mutation was an allele of the MRE11 gene and have renamed the mutation mre11-58. Two amino acid changes from the wild-type sequence were identified; one is located at a conserved site of a phosphodiesterase motif, and the other is a homologous amino acid change at a nonconserved site. Unlike mre11 null mutations, the mre11-58 mutation allowed meiosis-specific double-strand DNA breaks (DSBs) to form at recombination hot spots but failed to process those breaks. DSB ends of this mutant were resistant to lambda exonuclease treatment. These phenotypes are similar to those of rad50S mutants. In contrast to rad50S, however, mre11-58 was highly sensitive to methyl methanesulfonate treatment. DSB end processing induced by HO endonuclease was suppressed in both mre11-58 and the mre11 disruption mutant. We constructed a new mre11 mutant that contains only the phosphodiesterase motif mutation of the Mre11-58 protein and named it mre11-58S. This mutant showed the same phenotypes observed in mre11-58, suggesting that the phosphodiesterase consensus sequence is important for nucleolytic processing of DSB ends during both mitosis and meiosis.

References

Nov 1, 1991·Yeast·D R Higgins, J N Strathern
Jun 1, 1988·Molecular and Cellular Biology·B ConnollyJ E Haber
Sep 1, 1974·Mutation Research·J C Game, R K Mortimer
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R E Esposito, M S Esposito
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Oct 1, 1981·Molecular and Cellular Biology·R E Malone, R E Esposito
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Keeney, N Kleckner
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·T OgawaH Ogawa
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A WhiteT D Petes
Aug 1, 1995·Current Genetics·O V ChepurnayaV G Korolev
Oct 1, 1995·Trends in Biochemical Sciences·A Shinohara, T Ogawa
May 1, 1997·Molecular and Cellular Biology·P FiorentiniL S Symington
Mar 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J C Connelly, D R Leach
Feb 1, 1963·Genetics·F SHERMAN, H ROMAN
Jan 1, 1983·Molecular & General Genetics : MGG·R E Malone

❮ Previous
Next ❯

Citations

Feb 18, 2016·Seminars in Cell & Developmental Biology·Seoyoung KimScott Keeney
Nov 4, 2010·DNA Repair·Rebecca E KeelagherRhona H Borts
Jul 3, 2010·Science China. Life Sciences·Li Cui, Wei Li
Apr 28, 2009·DNA Repair·Sushmita MukherjeeJeff Sekelsky
Apr 21, 2009·Trends in Biochemical Sciences·Eleni P Mimitou, Lorraine S Symington
Jul 5, 2008·DNA Repair·Sylvia SteiningerSimone Moertl
Feb 26, 2008·DNA Repair·Alicia F LamLorraine S Symington
Oct 27, 2007·DNA Repair·Maria Pia LongheseMichela Clerici
Feb 27, 2007·Biochemical and Biophysical Research Communications·Satoshi KawashimaMasayuki Seki
Mar 4, 2011·The New Phytologist·Kim OsmanF Chris H Franklin
Oct 12, 2004·DNA Repair·Phuoc T TranR Michael Liskay
Jul 22, 2006·Experimental Cell Research·Takehiko UsuiMonica Morales
Jul 2, 2005·DNA Repair·Bridget WilliamsArthur J Lustig
Nov 2, 2015·Chromosoma·Jonathan RibeiroEmmanuelle Martini
Nov 13, 2004·FEMS Microbiology Reviews·Zuzana DudásováMiroslav Chovanec
Feb 13, 2007·Current Biology : CB·Sheila M CherryJohn H J Petrini
Jul 15, 1998·Current Biology : CB·D T Weaver
Aug 30, 2001·Current Biology : CB·P Kerr, A Ashworth
Oct 26, 2001·Current Biology : CB·A KegelS U Aström
Feb 18, 2004·Molecular Cell·Valérie BordeAlain Nicolas
May 29, 2003·Molecular Cell·José Antonio TerceroJohn F X Diffley
Oct 31, 2006·Molecular Cell·Christopher J FrankCarol W Greider
Jun 30, 2001·Molecular Cell·T UsuiJ H Petrini
May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Matteo VillaMaria Pia Longhese
Jun 1, 2016·Genomics, Proteomics & Bioinformatics·Ting Liu, Jun Huang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.