A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression

Scientific Reports
Chia-Hsun HsiehChun-Ta Liao

Abstract

The study of miRNAs and their roles as non-invasive biomarkers has been intensely conducted in cancer diseases over the past decade. Various platforms, ranging from conventional qPCRs to Next Generation Sequencers (NGS), have been widely used to analyze miRNA expression. Here we introduced a novel platform, PanelChip™ Analysis System, which provides a sensitive solution for the analysis of miRNA levels in blood. After conducting miRQC analysis, the system's analytical performance compared favorably against similar nanoscale qPCR-based array technologies. Because PanelChip™ requires only a minimal amount of miRNA for analysis, we used it to screen for potential diagnostic biomarkers in the plasma of patients with oral cavity squamous cell carcinoma (OSCC). Combining the platform with a machine learning algorithm, we were able to discover miRNA expression patterns capable of separating healthy subjects from patients with OSCC.

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Citations

Sep 29, 2019·BioTechniques·Diego A ForeroGeorge E Barreto
Apr 25, 2019·Nature Biomedical Engineering·Varun L KopparthyDavid Erickson

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Methods Mentioned

BETA
chip
Chips
PCR
blood draw
Assay
scraping

Software Mentioned

[UNK] Analysis System System
OpenArray®
[UNK] Analysis System
LIBSVM
miRQC
in R package

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