Apr 13, 2020

Activation of cytoplasmic dynein through microtubule crossbridging

BioRxiv : the Preprint Server for Biology
M. ChakrabortyAnne Straube


Cytoplasmic dynein is the main microtubule-minus-end-directed transporter of cellular cargo in animal cells. Cytoplasmic dynein also functions in the organisation and positioning of mitotic spindles and the formation of ordered microtubule arrays in neurons and muscle. Activation of the motor for cargo transport is thought to require formation of a complex with dynactin and a cargo adapter. Here we show that recombinant human dynein can crossbridge neighbouring microtubules and can be activated by this crossbridging to slide and polarity-sort microtubule bundles. While single molecules of human dynein are predominantly static or diffusive on single microtubules, they walk processively for 1.5 m on average along the microtubule bundles they form. Speed and force output of dynein are doubled on bundles compared to single microtubules, indicating that the crossbridging dynein steps equivalently on two microtubules. Our data are consistent with a model of autoactivation through the physical separation of dynein motor domains when crossbridging two microtubules. This enables cytoplasmic dynein to function effectively as a microtubule organiser and transporter without needing to first form a complex with dynactin and a cargo adapter.

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Immune System
Pulmonary Arterial Hypertension
Esophageal Tissue
Pathogenic Organism
Transcription, Genetic
Tissue Specificity

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.