A novel multiple FISH array for the detection of genetic aberrations in cancer

Laboratory Investigation; a Journal of Technical Methods and Pathology
Ruiyun LiFeng Jiang

Abstract

Interphase multicolor fluorescence in situ hybridization (IM-FISH) has great promise for improving cancer diagnosis because it can directly visualize multiple changes in chromosomes and gene copy number on a cell-to-cell basis. However, no more than four targets can be detected simultaneously by current commercially available IM-FISH protocols, and the DNA probes used are too large to detect the single-gene aberrations that characterize tumorigenesis. As a result, multiple FISH has a low sensitivity in detecting cancer cells. To overcome such limitations, we first developed specific genomic probes for the genes relevant to primary lung cancer. We next designed a multiple FISH array by arranging four different compositions of cocktails of four probes for each gene on a coverslip, which allowed four four-color FISH experiments to be performed in parallel on a single slide. We then tested the multiple FISH array on bronchial brushing samples from lung cancer patients to determine its ability to detect genetic abnormalities. A comparison of the data with the results of cytology and commercial four-color FISH suggested that the multiple FISH array had the highest sensitivity for cancer detection. The technique may thus be a powerful...Continue Reading

References

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Jul 13, 2001·Advances in Cancer Research·G Dölken
Feb 21, 2002·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Feng Jiang, Ruth L Katz
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Aug 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Feng JiangRuth L Katz

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Citations

Apr 28, 2012·Journal of Cancer Research and Clinical Oncology·Yi-Zhen LiuMing-Rong Wang
Nov 17, 2012·Expert Review of Molecular Diagnostics·Jesse S VossKevin C Halling
Sep 25, 2010·Cancer Prevention Research·Feng JiangSanford A Stass
Jan 27, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ruiyun LiFeng Jiang

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