A Novel Murine Chronic Obstructive Pulmonary Disease Model and the Pathogenic Role of MicroRNA-21

Frontiers in Physiology
Shengyang HeLihua Xie

Abstract

Chronic obstructive pulmonary disease (COPD) is a multi-pathogenesis chronic lung disease. The mechanisms underlying COPD have not been adequately illustrated. Many reseachers argue that microRNAs (miRs) could play a crucial role in COPD. The classic animal model of COPD is both time consuming and costly. This study proposes a novel mice COPD model and explores the role of miR-21 in COPD. A total of 50 wide-type (WT) C57BL/6 mice were separated into five euqlly-sized groups-(1) control group (CG), (2) the novel combined method group (NCM, cigarette smoke (CS) exposure for 28 days combined with cigarette smoke extract (CSE) intraperitoneal injection), (3) the short-term CS exposure group (SCSE, CS exposure for 28 days), (4) the CSE intraperitoneal injection group (CSEII, 28 days CSE intraperitoneal injection), and (5) the long-term CS exposure group (LCSE, CS exposure).The body weight gain of mice were recorded and lung function tested once the modeling was done. The pathological changes and the inflammation level by hematoxylin eosin (H&E) staining and immunohistochemical staining (IHS) on the lung tissue sections were also evaluated. The level of miR-21 in the mice lungs of the mice across all groups was detected by RT-qPCR an...Continue Reading

References

Jun 5, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·UNKNOWN World Medical Association General Assembly.
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·UNKNOWN World Medical Association, UNKNOWN American Physiological Society
Jan 11, 2003·American Journal of Respiratory and Critical Care Medicine·Kang-Hyeon ChoeNorbert F Voelkel
Sep 30, 2003·Thorax·A AgustíM Cosio
Nov 26, 2004·American Journal of Respiratory and Critical Care Medicine·Laimute Taraseviciene-StewartNorbert F Voelkel
Jun 10, 2005·Nature·Jun LuTodd R Golub
Aug 10, 2005·Genome Biology·Silvia MonticelliKenneth S Kosik
Jun 9, 2006·Journal of Occupational Medicine and Toxicology·Ulrich WagnerDavid A Groneberg
Aug 22, 2006·Proceedings of the American Thoracic Society·Laimute Taraseviciene-StewartNorbert F Voelkel
Oct 25, 2006·The Journal of Experimental Medicine·Bradley S CobbMatthias Merkenschlager
Sep 4, 2007·Lancet·Leonardo M Fabbri, Klaus F Rabe
Oct 11, 2007·PloS One·Haoquan WuN Manjunath
May 6, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joanne L WrightAndrew Churg
Jun 13, 2008·Nature·Brandi N DavisAkiko Hata
Feb 10, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yan ChenKeishi Kubo
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas X LuMarc E Rothenberg
Jun 6, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Shingen MisakaShizuo Yamada
Jul 21, 2009·Biochemical Society Transactions·S Duygu SelcukluCharles Spillane
Apr 3, 2010·Respiratory Medicine·Jordi OlloquequiJosé García-Valero
Dec 7, 2010·Expert Review of Respiratory Medicine·Joanne L Wright, Andrew Churg
Dec 27, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rebecca M BaronAugustine M K Choi
Jun 21, 2013·Frontiers in Oncology·Gianpiero Di Leva, Carlo M Croce
Oct 15, 2013·European Journal of Pharmacology·Masoumeh E GiviEsmaeil Mortaz
Feb 25, 2014·The Journal of Pharmacology and Experimental Therapeutics·T PeraH Maarsingh
Mar 1, 2014·Experimental & Molecular Medicine·Yukun ZhangHongtao Tian
Mar 13, 2014·Pneumonologia i alergologia polska·Maciej Kupczyk, Piotr Kuna
Feb 18, 2015·Frontiers in Immunology·Frederick J Sheedy
Oct 2, 2015·The Lancet. Respiratory Medicine·Guy G Brusselle, Ken R Bracke
Jun 17, 2016·COPD·Sanjay S Gautam, Ronan F O'Toole
Jan 28, 2017·American Journal of Respiratory and Critical Care Medicine·Claus F VogelmeierAlvar Agustí
Mar 9, 2017·Scandinavian Journal of Immunology·J LuS Sun
Apr 6, 2017·Experimental Lung Research·Hwa Young LeeSook Young Lee
Sep 15, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Dongxing ZhaoPixin Ran
Nov 2, 2017·International Journal of Chronic Obstructive Pulmonary Disease·Shengyang HeShenghua Sun

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