A novel murine long-chain acyl-CoA synthetase expressed in brain participates in neuronal cell proliferation

Biochemical and Biophysical Research Communications
Hae Jin KeeKyung Keun Kim

Abstract

Refsum disease (RfD) is an autosomal recessive neurologic disorder of the lipid metabolism. We have identified a novel murine long-chain acyl-CoA synthetase (mLACS) associated with the RfD gene using yeast two-hybrid assay. Northern blot analyses revealed that mLACS was expressed mainly in the brain and testis. mLACS was highly expressed in the brain at 2 weeks after birth and maintained through adult life. Expressions of the brain-specific LACS family increased in the PC12 cells undergoing neurite outgrowth by nerve growth factor. mLACS preferentially catalyzed the formation of arachidonoyl-CoA more than palmitoyl-CoA or oleoyl-CoA in PC12 cells. Triacsin C, an inhibitor of LACS, suppressed the cell proliferation and decreased mLACS expression in parent PC12 cells, but not in stably anti-sense mLACS cDNA-transfected cells. Our results indicate that mLACS participates in neuronal cell proliferation and differentiation, and interaction of the RfD gene with brain-selective mLACS may be involved in the pathogenesis of RfD.

References

Jul 12, 1996·The Journal of Biological Chemistry·T FujinoT Yamamoto
May 15, 1996·European Journal of Biochemistry·M KonoY Seyama
Nov 29, 1996·The Journal of Biological Chemistry·A UchiyamaT Hashimoto
Dec 1, 1996·European Journal of Biochemistry·H IijimaT Yamamoto
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M J KangT T Yamamoto
Nov 5, 1997·Nature Genetics·S J MihalikS J Gould
Nov 5, 1997·Nature Genetics·G A JansenR J Wanders
Apr 13, 1999·Biochemical and Biophysical Research Communications·S J SteinbergP A Watkins
Aug 22, 2000·European Journal of Human Genetics : EJHG·A S WierzbickiF B Gibberd
Dec 9, 2000·Biochemical and Biophysical Research Communications·A Moriya-SatoN Hashimoto
Jun 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·P Z TangM L Dufau

❮ Previous
Next ❯

Citations

Dec 3, 2014·Current Opinion in Immunology·Jessica F Brinkworth, Luis B Barreiro
Jun 4, 2016·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D R SeegerE J Murphy
Feb 14, 2007·Environmental Toxicology·Tetsuya MoriTadashi Kashiwagura
Feb 1, 2005·The Journal of Comparative Neurology·Tun-Tzu YuTimothy S McClintock
Oct 21, 2014·Journal of Proteome Research·Deivendran RengarajMyung-Geol Pang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.