A novel MVA-mediated pathway for isoprene production in engineered E. coli

BMC Biotechnology
Jianming YangHuizhou Liu

Abstract

To deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of great significance in that it is an important platform chemical, which has been used in industrial production of synthetic rubber for tires and coatings or aviation fuel. We firstly introduced fatty acid decarboxylase (OleTJE) from Jeotgalicoccus species into E. coli to directly convert MVA(mevalonate) into 3-methy-3-buten-1-ol. And then to transform 3-methy-3-buten-1-ol to isoprene, oleate hydratase (OhyAEM) from Elizabethkingia meningoseptica was overexpressed in E. coli. A novel biosynthetic pathway of isoprene in E. coli was established by co-expressing the heterologous mvaE gene encoding acetyl-CoA acetyltransferase/HMG-CoA reductase and mvaS gene encoding HMG-CoA synthase from Enterococcus faecalis, fatty acid decarboxylase (OleTJE) and oleate hydratase (OhyAEM). Furthermore, to enhance isoprene production, a further optimization of expression level of OleTJE, OhyAEM was carried out by using different promoters and copy...Continue Reading

References

Nov 8, 1965·Biochemical and Biophysical Research Communications·W G Niehaus, G J Schroepfer
Oct 2, 2002·Bioscience, Biotechnology, and Biochemistry·Tomohisa Kuzuyama
Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling
Jul 2, 2003·Current Opinion in Microbiology·Alexander Steinbüchel
Nov 1, 1962·Archives of Biochemistry and Biophysics·L L WALLENR W JACKSON
Jan 24, 2007·Metabolic Engineering·Douglas J PiteraJay D Keasling
Feb 10, 2007·Science·Gregory Stephanopoulos
Apr 27, 2007·Applied Microbiology and Biotechnology·Mattijs K JulsingOliver Kayser
May 26, 2009·Journal of Bacteriology·Loes E BeversWilfred R Hagen
Aug 19, 2009·Applied Biochemistry and Biotechnology·Jiashi WeiBaorong Lu
Jan 6, 2010·Nucleic Acids Research·Thomas E Kuhlman, Edward C Cox
Feb 10, 2010·Applied Microbiology and Biotechnology·James M Clomburg, Ramon Gonzalez
Jan 11, 2011·Applied and Environmental Microbiology·Mathew A RudeAndreas Schirmer
Apr 7, 2011·Applied Microbiology and Biotechnology·Yaru ZhaoMo Xian

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Citations

Jul 17, 2016·Applied and Environmental Microbiology·Suresh Chandra PhularaPratima Gupta
Dec 23, 2016·The Journal of Biological Chemistry·Kento MotoyamaHisashi Hemmi
Jul 14, 2020·Chembiochem : a European Journal of Chemical Biology·Daniel BauerVolker Sieber
Jan 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xueying ZhangJiyan Zhang
Jul 15, 2018·Applied Microbiology and Biotechnology·Meijie LiHaibo Zhang
Jan 5, 2021·Applied Microbiology and Biotechnology·Govinda R NavaleSandip S Shinde
Jul 8, 2021·Natural Product Reports·Mauro A RinaldiNigel S Scrutton

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Methods Mentioned

BETA
PCR
Protein Assay

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