A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia.

The Journal of Biological Chemistry
B C Kone, S C Higham

Abstract

The H+-K+-ATPase of renal collecting duct mediates K+ conservation during chronic hypokalemia. K+ deprivation promotes H+-K+-ATPase alpha2 (HKalpha2) gene expression in the medullary collecting duct, the principal site of active K+ reabsorption, suggesting that this isozyme contributes to renal K+ reclamation. We report here that alternative transcriptional initiation and mRNA splicing give rise to distinct N-terminal variants of the HKalpha2 subunit. Sequence analysis and in vitro translation revealed that HKalpha2a corresponds to the known HKalpha2 cDNA (Crowson, M. S., and Shull, G. E. (1992) J. Biol. Chem. 267, 13740-13748), whereas HKalpha2b represents a novel variant truncated by 108 amino acids at its N terminus. HKalpha2b mRNA contains a complex 5'-untranslated region with eight upstream open reading frames, features implicated in translational regulation of other genes. Heterologous expression of HKalpha2b with and without the gastric H+-K+-ATPase beta subunit in HEK 293 cells indicated that this variant encodes a K+ uptake mechanism that is relatively Sch 28080-resistant, partially sensitive to ouabain, and appears to require coexpression with the gastric H+-K+-ATPase beta subunit for optimal functional activity. Nort...Continue Reading

References

Oct 1, 1992·Molecular and Cellular Biology·H Y Yang, T Evans
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J A MolitorW C Greene
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R H CostaJ E Darnell
Jan 22, 1982·Nucleic Acids Research·S M Mount
Oct 1, 1995·The American Journal of Physiology·N N ModyanovK Geering
Oct 1, 1995·The Journal of Clinical Investigation·J LeeH J Binder
May 1, 1995·The American Journal of Physiology·B G BachhuberG K Owens
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·O I Shamraj, J B Lingrel
Mar 29, 1996·The Journal of Biological Chemistry·M CougnonF Jaisser
Apr 1, 1996·The American Journal of Physiology·A GreshamA P Pentland
Jan 1, 1997·The American Journal of Physiology·D A EssigD A Jackson

❮ Previous
Next ❯

Citations

Oct 7, 2005·Pflügers Archiv : European journal of physiology·Olivier DherbecourtAlain Doucet
Feb 10, 2010·Pflügers Archiv : European journal of physiology·Mads V SorensenJens Leipziger
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·J Preben MorthPoul Nissen
Nov 7, 2001·The Biochemical Journal·J NishiyamaZ Dong
Sep 1, 1999·Kidney International·G Fejes-TóthH Velázquez
Oct 28, 2010·The Journal of Biological Chemistry·Ivana NovakSusanne E Hede
Dec 14, 1999·Current Opinion in Nephrology and Hypertension·T D DuBoseJ Codina
Mar 17, 2004·Annals of Surgery·Kenneth S HelmerDavid W Mercer
Dec 1, 2004·Kidney International·Juan CodinaThomas D Dubose
Jul 31, 2009·American Journal of Physiology. Renal Physiology·Michelle L GumzCharles S Wingo
Jan 5, 2002·Biological Chemistry·A AbudulaB Wiederanders
Jul 7, 2007·Expert Opinion on Therapeutic Targets·Deborah L ZiesBrian D Cain
Oct 31, 2006·Seminars in Nephrology·Juan Codina, Thomas D DuBose
May 13, 2008·Biochemical and Biophysical Research Communications·Scott J RobertsColin Farquharson
Oct 13, 2006·Biochimica Et Biophysica Acta·Deborah L ZiesBrian D Cain
Feb 12, 2004·American Journal of Physiology. Renal Physiology·Wenzheng ZhangBruce C Kone
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Doris StreifMarkus Ritter
Jan 30, 2004·American Journal of Physiology. Cell Physiology·Nikolay B PestovNikolai N Modyanov
Jul 28, 2005·The Journal of Biological Chemistry·Herman G P SwartsJan Joep H H M De Pont
Jan 17, 2014·American Journal of Physiology. Renal Physiology·Gilles Crambert
Oct 17, 1998·The Journal of Biological Chemistry·A V Grishin, M J Caplan
Jun 11, 1999·The American Journal of Physiology·F Jaisser, A T Beggah
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Nikolay B PestovNikolai N Modyanov
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Gilles CrambertKäthi Geering
Nov 30, 2000·American Journal of Physiology. Renal Physiology·N Laroche-JoubertA Doucet
Jun 11, 1999·The American Journal of Physiology·R B Silver, M Soleimani
Jul 18, 2001·American Journal of Physiology. Renal Physiology·G Fejes-Tóth, A Náray-Fejes-Tóth
Apr 18, 2015·Journal of Veterinary Emergency and Critical Care·Molly A Weingarten, Allison A Sande
Sep 13, 2001·American Journal of Physiology. Renal Physiology·J A KrautG Sachs
Feb 9, 2000·American Journal of Physiology. Renal Physiology·A E FrankI D Weiner
Jul 12, 2002·American Journal of Physiology. Renal Physiology·J ReinhardtH Oberleithner
Oct 1, 1998·The American Journal of Physiology·S M WallT D DuBose
Jul 18, 2001·American Journal of Physiology. Renal Physiology·T KuncewiczB C Kone
Sep 8, 1998·The American Journal of Physiology·J CodinaT D DuBose
Oct 13, 2000·American Journal of Physiology. Cell Physiology·J CodinaT D Dubose
Mar 4, 2008·The FEBS Journal·Simona TavernaMaria Letizia Vittorelli
Feb 10, 1999·The American Journal of Physiology·W G CampbellB D Cain
Jul 18, 2001·American Journal of Physiology. Renal Physiology·J W VerlanderC S Wingo
Apr 13, 1999·The American Journal of Physiology·I D WeinerC S Wingo
Apr 13, 1999·The American Journal of Physiology·B C Kone, S Higham
Aug 26, 1998·Lancet·S L Gluck
Dec 1, 2001·Journal of the American Society of Nephrology : JASN·Wenzheng ZhangBruce C Kone
Feb 4, 2010·Archives of Biochemistry and Biophysics·Dimas C BelisarioJesús R del Castillo

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.