A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus

Neuroscience
T MatsushitaS Hashimoto

Abstract

The suprachiasmatic nucleus of the anterior hypothalamus is the center of an internal biological clock in mammals. Glutamate is the neurotransmitter of retino-hypothalamic tract responsible for mediating the circadian actions of light in rodents. N-methyl-d-aspartate receptors, particularly NR2B subunit are reported to be principally involved in photic resetting of the biological clock in vivo and in slice culture. But, the precise cellular mechanisms of the resetting are not elucidated, because no adequate neuronal cell lines derived from the suprachiasmatic nucleus have been established. We established a neuronal cell line, N14.5, derived from the suprachiasmatic nucleus of a transgenic rat harboring the temperature-sensitive simian virus 40 large T-antigen gene. When the cells were cultured at 39 degrees C, the morphological features were turned fibroblastic into neuronal round cell body with neurite extensions. These cells showed immunoreactivities for neuronal markers (betaIII-tubulin, microtubule-associated protein 2 and TAU2) and as well as for vasoactive intestinal peptide which is expressed in the ventrolateral region of the suprachiasmatic nucleus. The cells expressed N-methyl-d-aspartate receptors, particularly NR1 a...Continue Reading

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Citations

Sep 24, 2008·Journal of Molecular Neuroscience : MN·Camilla P PretzmannBirgitte Georg
Apr 4, 2009·Frontiers in Neuroendocrinology·Christopher M MayerDenise D Belsham
Feb 20, 2007·Biochemical and Biophysical Research Communications·Soshi KawaguchiHajime Tei

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