A Novel NGF Receptor Agonist B355252 Ameliorates Neuronal Loss and Inflammatory Responses in a Rat Model of Cerebral Ischemia.

Journal of Inflammation Research
Hao-Kuang WangCheng-Chun Wu

Abstract

Cerebral ischemia is a leading cause of disability and death worldwide. However, an effective therapeutic approach for the condition remains undiscovered. The previously proposed growth factor-based therapy has been inefficient due to its inability to pass through the blood-brain barrier. B355252, a newly developed small molecule, exhibited a potential neuroprotective effect in vivo. However, its exact efficacy in cerebral ischemia remains unclear. We adopt an endothelin-1 stereotaxic intracranial injection to induced cerebral ischemia in rat. We further conducted 2,3,5-triphenyltetrazolium chloride (TTC) staining, immunofluorescent staining, enzyme-linked immunosorbent assay (ELISA), and behavioral tests to evaluate the efficacy of B355252 in neuroprotection, anti-inflammation, and behavioral outcome improvements. We identified that B355252 could protect ischemic neurons from neuronal loss by attenuating DNA damage, reducing ROS production and the LDH level, and preventing neuronal apoptosis. Moreover, inflammatory responses in astrocytic and microglial gliosis, as well as IL-1β and TNF-α levels, were ameliorated. Consequently, the behavioral outcomes of ischemic rats in neurologic responses and fore paw function recovery were...Continue Reading

References

Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P B EhrhardU Otten
Dec 2, 1999·Experimental Cell Research·W J Friedman, L A Greene
Feb 26, 2000·Trends in Pharmacological Sciences·H U Saragovi, K Gehring
Jun 14, 2000·Current Opinion in Neurobiology·D R Kaplan, F D Miller
Aug 10, 2000·Gastroenterology·M ReinshagenG Adler
Dec 29, 2000·Journal of Chemical Information and Computer Sciences·T Osterberg, U Norinder
Feb 13, 2001·Journal of Neuroimmunology·M C CaroleoL Aloe
Jun 12, 2001·Current Opinion in Neurobiology·A Patapoutian, L F Reichardt
Aug 25, 2001·Annual Review of Neuroscience·E J Huang, L F Reichardt
Jun 18, 2002·Journal of Neurochemistry·Lone DagøMette Grønborg
Aug 17, 2002·Science·Greg Miller
Oct 5, 2002·Stroke; a Journal of Cerebral Circulation·Ya HuaGuohua Xi
Jul 16, 2003·Trends in Immunology·Taro Fukao, Shigeo Koyasu
Apr 29, 2005·Current Drug Targets. CNS and Neurological Disorders·Jing Mei Ren, Seth P Finklestein
Jun 2, 2005·Nature Reviews. Immunology·Foo Y LiewLuke A J O'Neill
Dec 22, 2005·Nature Immunology·Jiahuai Han, Richard J Ulevitch
Feb 21, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Hassan Pajouhesh, George R Lenz
Sep 21, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bryan KolbSamuel Weiss
Dec 26, 2006·Journal of Neuroimmunology·Qing WangMidori A Yenari
Aug 19, 2007·Current Neurovascular Research·Aurel Popa-WagnerLary C Walker
Nov 22, 2007·Trends in Molecular Medicine·Taro Kawai, Shizuo Akira
Jul 16, 2008·Journal of Neuroscience Methods·Nobutaka HorieGary K Steinberg
Apr 23, 2009·Journal of Neuroscience Methods·Kelly A Tennant, Theresa A Jones
May 26, 2009·Brain Research·Ludmila BelayevSteven C Cramer
Dec 18, 2009·Journal of Cellular and Molecular Medicine·S LanfranconiA Bersano
Jul 21, 2010·Experimental & Translational Stroke Medicine·Krystal L SchaarSean I Savitz
Dec 9, 2010·Brain Research·Tiziana GenoveseSalvatore Cuzzocrea
Jul 13, 2011·The Journal of Clinical Investigation·Boyeon LeeGregory J Bix
Sep 15, 2012·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Laura M AllenHamed Farid
Oct 10, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sophia L B OliveiraHenning Ulrich
Mar 9, 2013·CNS & Neurological Disorders Drug Targets·Ramón RodrigoWalter Feuerhake
Oct 23, 2013·Behavioural Brain Research·Chris A CordovaDale Corbett
Mar 4, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giusi PrencipeLuisa Bracci-Laudiero
May 9, 2014·PloS One·Hima C S AbeysingheCarli L Roulston
Feb 5, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Francesco BlasiCenk Ayata
Dec 10, 2015·Cellular and Molecular Neurobiology·Nailya S Gliyazova, Gordon C Ibeanu
May 29, 2018·Pharmacology & Therapeutics·Zhenhua ZhouQin Hu

❮ Previous
Next ❯

Citations

Aug 31, 2021·Cellular and Molecular Neurobiology·Bhakta Prasad Gaire

❮ Previous
Next ❯

Software Mentioned

ImageJ
ARRIVE

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.