A novel nicotinamide adenine dinucleotide control strategy for increasing the cell density of Haemophilus parasuis

Biotechnology Progress
Likun ChengZhiqiang Shen

Abstract

Haemophilus parasuis is the causative agent of Glässer's disease and is a major source of economic losses in the swine industry each year. To enhance the production of an inactivated vaccine against H. parasuis, the availability of nicotinamide adenine dinucleotide (NAD) must be carefully controlled to ensure a sufficiently high cell density of H. parasuis. In the present study, the real-time viable cell density of H. parasuis was calculated based on the capacitance of the culture. By assessing the relationship between capacitance and viable cell density/NAD concentration, the NAD supply rate could be adjusted in real time to maintain the NAD concentration at a set value based on the linear relationship between capacitance and NAD consumption. The linear relationship between cell density and addition of NAD indicated that 7.138 × 109 NAD molecules were required to satisfy per cell growth. Five types of NAD supply strategy were used to maintain different NAD concentration for H. parasuis cultivation, and the results revealed that the highest viable cell density (8.57, OD600 ) and cell count (1.57 × 1010 CFU/mL) were obtained with strategy III (NAD concentration maintained at 30 mg/L), which were 1.46- and 1.45- times more, respe...Continue Reading

References

Apr 12, 2000·Molecular Microbiology·J ReidlE Soleva
Mar 17, 2004·Veterinary Microbiology·Simone Oliveira, Carlos Pijoan
Jun 2, 2005·Biotechnology Letters·M H SarrafzadehC Ghommidh
May 24, 2006·Microbial Cell Factories·Franz Clementschitsch, Karl Bayer
Sep 15, 2006·Trends in Biotechnology·Mark A Eiteman, Elliot Altman
May 10, 2012·Applied Microbiology and Biotechnology·Moira Monika Schuler, Ian William Marison
Nov 28, 2012·Bioprocess and Biosystems Engineering·Patrick WechselbergerChristoph Herwig
Jul 26, 2013·Clinical and Vaccine Immunology : CVI·Susan L BrockmeierMarcus E Kehrli
Nov 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yasmeen RahimiGerald I Shulman
Mar 27, 2015·Letters in Applied Microbiology·W GuZ X Ji
Feb 6, 2017·Microbial Cell Factories·Kati ThielPauli Tapio Kallio
Aug 9, 2017·Biotechnology Letters·Paul KrollChristoph Herwig
Sep 28, 2017·Biochimica Et Biophysica Acta. Bioenergetics·Kristin A AndersonMatthew D Hirschey

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