A novel non-destructive technique for qualitative and quantitative measurement of dental erosion in its entirety by porosity and bulk tissue-loss.

Journal of Dentistry
Mathew J F HookhamDeclan P Naughton

Abstract

To explore the potential of combining non-contact profilometry (NCP) and confocal laser scanning microscopy (CLSM) data to measure the entire erosive process non-destructively and to validate findings using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), scanning electron microscopy (SEM) and surface microhardness (SMH) using the same samples throughout. Polished bovine enamel samples (n = 35) were divided into groups (7/group) with similar SMH values. Samples underwent individual erosive challenges (1 % citric acid, pH3.8) for 1, 5, 10, 15 or 30 min under stirring and aliquot extracts were analysed for Ca and P by ICP-AES. SMH was used to measure erosive softening. Profilometry was used to assess bulk volume loss (BVL). Images were captured by SEM. Samples were stained with rhodamine-B (0.1 mM, 24 h) and images captured by CLSM. Image processing was used to determine changes in fluorescent volume for the first 10 μm (ΔFV10) for each enamel sample which were combined with BVL to calculate total lesion volume (TLV). ANOVA, linear regression and Pearson correlation analysis were used where applicable. Surface softening, [Ca], [P], BVL and ΔFV10μm increased with acid erosion duration which were significant by 10...Continue Reading

References

Feb 21, 1998·Advances in Dental Research·A F HallS L Creanor
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C RobinsonJ Kirkham
Feb 28, 2003·International Journal of Paediatric Dentistry·J H NunnA Walker
Sep 26, 2008·Chemical Reviews·Lijun Wang, George H Nancollas
Jan 6, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Erik HeurichBernd W Sigusch
Jan 26, 2010·Acta Odontologica Scandinavica·Nadine SchlueterCarolina Ganss
Mar 9, 2012·Bioinorganic Chemistry and Applications·Kensuke Kuroda, Masazumi Okido
Nov 14, 2012·Journal of Dentistry·J GomezR Ellwood
Apr 8, 2014·Microscopy Research and Technique·Ana Marly Araújo MaiaJohn Michael Girkin
Jun 25, 2015·Brazilian Oral Research·Amnah Abdullah AlgarniAnderson Takeo Hara
Sep 26, 2015·British Dental Journal·J J MurrayR D Holmes
May 6, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·F MullanR S Austin
Aug 18, 2017·PloS One·Gabriela Cristina de OliveiraDaniela Rios
Oct 27, 2017·Journal of Healthcare Engineering·Monika MachoyKrzysztof Woźniak
Jan 13, 2018·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·M Skalsky JarkanderK Carlstedt
Aug 23, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·P MylonasD W Bartlett
Jan 1, 2020·Journal of Dentistry·Sultan E AinoosahFrank Lippert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.