A novel obligate intracellular gamma-proteobacterium associated with ixodid ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov.

PloS One
Oleg MediannikovDidier Raoult

Abstract

Obligate intracellular bacteria of arthropods often exhibit a significant role in either human health or arthropod ecology. An obligate intracellular gamma-proteobacterium was isolated from the actively questing hard tick Ixodes ricinus using mammalian and amphibian cell lines. Transmission electron microscopy revealed a unique morphology of the bacterium, including intravacuolar localization of bacteria grouped predominantly in pairs and internal structures composed of electron-dense crystal-like structures and regular multilayer sheath-like structures. The isolate 20B was characterized to determine its taxonomic position using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that this strain belongs to the family Coxiellaceae, order Legionellales of Gamma-proteobacteria, and the closest relatives are different Rickettsiella spp. The level of 16S rRNA gene sequence similarity between strain 20B and other recognized species of the family was below 94.5%. Partial sequences of the rpoB, parC and ftsY genes confirmed the phylogenetic position of the new isolate. The G+C content estimated on the basis of whole genome analysis of strain 20B was 37.88%. On the basis of its phenotypic and genotypic properties,...Continue Reading

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Citations

Nov 28, 2012·Ticks and Tick-borne Diseases·Oleg MediannikovDidier Raoult
Jul 26, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G SubramanianD Raoult
Nov 28, 2015·Experimental & Applied Acarology·Radek KlubalJan Hubert
Nov 13, 2012·Ticks and Tick-borne Diseases·M Pilar AlberdiLesley Bell-Sakyi
Jun 30, 2012·Ticks and Tick-borne Diseases·M Pilar AlberdiLesley Bell-Sakyi
Nov 10, 2011·FEMS Immunology and Medical Microbiology·Geetha SubramanianOleg Mediannikov
Dec 1, 2011·FEMS Immunology and Medical Microbiology·Mark TaylorGilbert Greub
Feb 24, 2015·Folia Microbiologica·Daniele Corsaro, Danielle Venditti
Dec 18, 2013·Applied and Environmental Microbiology·Clare A Anstead, Neil B Chilton
Jun 19, 2013·Applied and Environmental Microbiology·Yoshiko IshiiTakema Fukatsu
Jan 9, 2015·Clinical Microbiology Reviews·Jean-Christophe LagierDidier Raoult
Apr 21, 2020·Emerging Infectious Diseases·Carter MerensteinDavid Allen
Oct 24, 2020·Insects·Joyce Megumi SakamotoElizabeth Anne Wagner
May 7, 2013·Applied and Environmental Microbiology·Lilach Iasur-KruhEinat Zchori-Fein

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Datasets Mentioned

BETA
AAQJ00000000
CP000947.1
CP000675.2

Methods Mentioned

BETA
transmission electron microscopy
GTPase
PCR

Software Mentioned

BLAST
MEGA3

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