PMID: 7539483Jan 1, 1995Paper

A novel omega-conopeptide for the presynaptic localization of calcium channels at the mammalian neuromuscular junction

Journal of Neurocytology
Y SugiuraC P Ko

Abstract

Voltage-sensitive Ca2+ channels are essential to transmitter release at the chemical synapse. To demonstrate the localization of voltage-sensitive Ca2+ channels in relation to the site of transmitter release, mouse neuromuscular junctions were double labelled with alpha-bungarotoxin and a novel voltage-sensitive Ca2+ channel probe, SNX-260, a synthetic analog of omega-conopeptide MVIIC. Similar to omega-conopeptide MVIIC, biotinylated SNX-260 blocked nerve-stimulated transmitter release at the mouse neuromuscular junction. Fluorescently-tagged biotinylated SNX-260 labelled the nerve terminal which appeared thinner than and was outlined by acetylcholine receptor clusters as seen in en face view. This SNX-260 labelling was inhibited by preincubation with unconjugated SNX-260. Side-views of the neuromuscular junction indicated that the SNX-260 labelling was on the synaptic side facing the acetylcholine receptor rather than on the nonsynaptic side of the nerve terminal. This presynaptic binding was confirmed by the absence of SNX-260 labelling in denervated muscles following a nerve cut or disjunction after collagenase treatment. Confocal microscopy revealed spots of SNX-260 labelling that may correlate with active zones. The SNX-2...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·O D UchitelR Llinás
Oct 20, 1992·Biochemistry·C A RamiloB M Olivera
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·A De LucaD F Story
Jan 1, 1991·Annual Review of Neuroscience·W S TrimbleR H Scheller
Feb 1, 1986·Pflügers Archiv : European journal of physiology·R Penner, F Dreyer
Jan 1, 1987·Annual Review of Neuroscience·G J AugustineS J Smith
Oct 1, 1988·Journal of Electron Microscopy Technique·J E RashM Morita
Feb 27, 1968·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R Miledi, C R Slater
Mar 1, 1974·Journal of Neurocytology·J E HeuserD M Landis
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D W PumplinR Llinás
May 1, 1984·The Journal of Cell Biology·C P Ko
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H FukunagaA Vincent
Mar 15, 1984·Nature·L M Kerr, D Yoshikami
Jan 1, 1981·Toxicon : Official Journal of the International Society on Toxinology·C ClarkL J Cruz
Mar 1, 1981·Biophysical Journal·R LlinásK Walton
Jan 1, 1994·Annual Review of Biochemistry·B M OliveraM E Adams
Nov 1, 1993·Neuropharmacology·V D MonjeW R Gray
Jul 9, 1971·Nature·B NagyH C Urey

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Citations

Sep 1, 1995·Progress in Neurobiology·W M Fu
Jul 28, 1995·Neuroscience Letters·M J Lin, S Y Lin-Shiau
Aug 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·O D Uchitel
Jun 5, 1998·Trends in Pharmacological Sciences·R A OphoffM D Ferrari
Dec 28, 1999·Neuroscience Letters·A AmbrosiniJ Schoenen
Jun 1, 1995·The New England Journal of Medicine·V A LennonE H Lambert
Apr 1, 1997·The European Journal of Neuroscience·M J Lin, S Y Lin-Shiau
Apr 15, 1999·Annual Review of Neuroscience·J R Sanes, J W Lichtman
Mar 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Francisco J UrbanoRichard W Tsien
Jan 11, 2007·Médecine sciences : M/S·Norbert WeissMichel De Waard
Nov 4, 2000·Expert Opinion on Investigational Drugs·K K Jain
Jul 6, 2010·Journal of Neuroscience Methods·Shannon ModlaRobert E Akins
Dec 4, 2012·Mechanisms of Development·John H CaldwellUlrike C Müller
Mar 12, 2008·The Journal of Hand Surgery, European Volume·S R SabapathyA S Ullah
Dec 29, 2019·Transboundary and Emerging Diseases·Abbas Aghaei AfsharAhmad Ali Hanafi-Bojd
Sep 29, 1998·The European Journal of Neuroscience·V MagnelliE Carbone
Jun 7, 1996·The Journal of Biological Chemistry·H LiuK P Campbell
Jul 21, 1998·Annals of the New York Academy of Sciences·M TakamoriK Komai
Jul 3, 1999·Physiological Reviews·A MeirR Rahamimoff
Aug 5, 2011·Physiological Research·L F NurullinE E Nikolsky
Aug 1, 1997·Annals of Neurology·S A WatermanJ Newsom-Davis

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