A novel on-line gold nanoparticle-catalyzed luminol chemiluminescence detector for high-performance liquid chromatography

Journal of Chromatography. a
Qun Lin ZhangXiao Yue Zhang

Abstract

A novel on-line gold nanoparticle-catalyzed luminol-H(2)O(2) chemiluminescence (CL) detector for high-performance liquid chromatography (HPLC) was established, in which gold nanoparticles were produced by the on-line reaction of H(2)O(2), NaHCO(3)-Na(2)CO(3) (buffer solution of luminol), and HAuCl(4). Eight phenolic compounds (gallic acid, protocatechuic acid, protocatechuic aldehyde, 2,5-dihydroxybenzoic acid, caffeic acid, 2,3-dihydroxybenzoic acid, (+)-catechin, and (-)-epicatechin) were chosen as the model compounds. Every separated phenolic compound in the column eluent strongly enhanced the CL signal of on-line gold nanoparticle-catalyzed luminol system. The CL and UV-visible absorption spectra and transmission electron microscopy studies were carried out, and the CL enhancement mechanism was ascribed to that the presence of phenolic compound promoted the on-line formation of 38-nm-diameter gold nanoparticles, which better catalyzed the luminol-H(2)O(2) CL reaction. The effects of methanol and phosphoric acid in the proposed HPLC configuration were performed by two gradient elution programs, and the baseline profile revealed that on-line gold nanoparticle-catalyzed luminol-H(2)O(2) CL detector had better compatibility tha...Continue Reading

Citations

Jun 9, 2014·Analytical and Bioanalytical Chemistry·Na LiHua Cui
Nov 3, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Selvaraj NaveenrajSambandam Anandan
Nov 20, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Dong WuLiuxin Xu

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