A novel PADRE-Kv1.3 vaccine effectively induces therapeutic antibodies and ameliorates experimental autoimmune encephalomyelitis in rats

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Cheng FanKun Liu

Abstract

Previous studies have confirmed that selective blockade of Kv1.3 channels could modulate the activities of pathogenic T cells and microglia/macrophages, which play key roles in experimental autoimmune encephalomyelitis (EAE). In this study, we designed an anti-Kv1.3 vaccine (PADRE-Kv1.3) to explore its protective role in EAE rat models. When the vaccine was applied in EAE rats, clinical scores and several staining techniques were used to evaluate the severity of the disease. T cell subtypes and related cytokines, as well as microglia/macrophage activation were assayed through flow cytometry, qRT-PCR or immunofluorescence staining, respectively. We herein showed that rats and mice developed high titers of anti-Kv1.3 antibodies and appeared no abnormal manifestations after the PADRE-Kv1.3 vaccine treatment. In EAE models, the vaccine treatment effectively alleviated the clinical severity and lessened pathological damages in the central nervous system (CNS). In addition, we found the vaccine significantly decreased the number of pathogenic T cells (Th17 and IFN-γ-producing T cells) and the production of related pro-inflammatory cytokines (IL-17A, IFN-γ and IL-1β), but increased the number of protective T subsets (CD4+IL-10+ T cell...Continue Reading

Citations

Feb 6, 2020·Vaccines·Dejan JakimovskiRobert Zivadinov
Aug 23, 2020·International Journal of Molecular Sciences·Maria V Pinto, Adelaide Fernandes
Dec 12, 2018·MAbs·Catherine J HutchingsTheodore G Clark
Apr 11, 2019·Frontiers in Pharmacology·Jiaying WangQiaojun He
Feb 18, 2020·Natural Product Reports·Saumya BajajK George Chandy
Apr 9, 2020·Frontiers in Immunology·Brooke L Guerrero, Nancy L Sicotte
Feb 8, 2019·Nature Reviews. Drug Discovery·Heike WulffVladimir Yarov-Yarovoy

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