A novel paraptosis pathway involving LEI/L-DNaseII for EGF-induced cell death in somato-lactotrope pituitary cells

Apoptosis : an International Journal on Programmed Cell Death
Joanna FombonneA Torriglia

Abstract

We have recently reported that EGF triggers an original form of cell death in pituitary cell line (GH4C1) with a phenotype sharing some characteristics of both apoptosis (internucleosomal DNA fragmentation) and paraptosis (caspase-independence and cytoplasmic vacuolization). However, the endonuclease involved in EGF-induced DNA fragmentation has not been assessed so far. In the present work we therefore further explored the putative paraptosis involvement in EGF-induced cell death and asked whether L-DNaseII might be involved. Indeed, this endonuclease is known to mediate internucleosomal DNA fragmentation in caspase independent manner. Our Western blot, immunocytochemistry and enzymatic measurement assays show that EGF triggers a cleavage of Leukocyte Elastase Inhibitor (LEI) precursor into L-DNaseII, its subsequent enzymatic activation and nuclear translocation thus pointing to the involvement of this endonuclease pathway in caspase-independent DNA fragmentation. In addition, EGF-induced cell death can be blocked by paraptosis inhibitor AIP-1/Alix, but not with its anti-apoptotic C-terminal fragment (Alix-CT). Altogether these data suggest that EGF-induced cell death defines a novel, L-DNaseII-mediated form of paraptosis.

References

Aug 1, 1987·The Journal of Clinical Endocrinology and Metabolism·P BirmanD Bression
Feb 7, 1997·Cell·S Nagata
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Mar 14, 2000·Cell Death and Differentiation·R A LockshinZ Zakeri
Aug 5, 2000·Biochemical and Biophysical Research Communications·P PeraniY Courtois
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S SperandioD E Bredesen
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·C ForcetP Mehlen
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·R A Lockshin, Z Zakeri
Jul 13, 2001·The Journal of Biological Chemistry·R V RaoD E Bredesen
May 30, 2002·The Journal of Biological Chemistry·Christine Chatellard-CausseRemy Sadoul
Jul 11, 2002·Cell Death and Differentiation·S Castro-ObregónD E Bredesen
Dec 11, 2002·Current Opinion in Cell Biology·Richard A Lockshin, Zahra Zakeri
Apr 30, 2003·Nature Immunology·Marja Jäättelä, Jürg Tschopp
Aug 23, 2003·Cell Death and Differentiation·Séverine AltairacAlicia Torriglia
Dec 4, 2003·Gene·Cory J Evans, Renato J Aguilera
Jun 15, 2004·Cell Death and Differentiation·S SperandioD E Bredesen
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Richard A Lockshin, Zahra Zakeri
Aug 28, 2004·Neuroscience Letters·Sabine ChahoryAlicia Torriglia
Oct 1, 2004·Molecular and Cellular Biology·Mirko H H SchmidtOliver Bögler

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Citations

Jun 10, 2011·Apoptosis : an International Journal on Programmed Cell Death·Xiaohong HuangQiwei Qin
May 16, 2013·Apoptosis : an International Journal on Programmed Cell Death·Chloé LeprêtreAlicia Torriglia
Jul 31, 2013·Apoptosis : an International Journal on Programmed Cell Death·Ibrahim AlanaziDavid L Adelson
Nov 4, 2011·Cell Death & Disease·A M WasikB Sander
Jun 30, 2009·The Journal of Biological Chemistry·Saverio TarditoRenata Franchi-Gazzola
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·N I Aleksandrushkina, B F Vanyushin
Jan 24, 2016·Pharmacology & Therapeutics·Dongjoo LeeKyeong Sook Choi
Jul 27, 2010·Biochemical Pharmacology·Alicia TorrigliaFrancine Behar-Cohen
Jan 9, 2008·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Hans K Lorenzo, Santos A Susin
Oct 24, 2015·Virus Research·Latif ReshiJiann-Ruey Hong
Feb 3, 2007·Progress in Neurobiology·Slavica KranticRémi Quirion
Sep 2, 2008·Biochemical Pharmacology·Alicia TorrigliaElisabeth Martin
Jan 26, 2016·Metallomics : Integrated Biometal Science·Melchior CiniTracey D Bradshaw
Sep 11, 2010·Journal of Cellular Biochemistry·Sabina SperandioDale E Bredesen
Nov 18, 2009·Journal of Cellular Physiology·Qingrui SunXunbin Wei
Dec 3, 2014·Biochemical and Biophysical Research Communications·Si Hyoung KimSeong Jin Lee
Sep 29, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mónica Suárez KorsnesLene C Hermansen
Mar 18, 2008·Biochimica Et Biophysica Acta·Chloé LeprêtreAlicia Torriglia
Jun 7, 2008·Journal of Medical Entomology·Julia W PridgeonKenneth J Linthicum
Jun 23, 2016·Oncotarget·Andrey V ShubinSergey V Kostrov

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