A Novel Particulate Delivery System Based on Antigen-Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus.

Molecular Pharmaceutics
Shuai LiSongping Zhang

Abstract

The interactions between antigen and adjuvant were among the most significant factors influencing the immunogenicity of vaccines, especially for unstable antigens like inactivated foot and mouth disease virus (iFMDV). Here we propose a novel antigen delivery pattern based on the coordination interaction between transition metal ions Zn2+ chelated to chitosan nanoparticles and iFMDV, which is known to be rich in histidine. The zinc chelated chitosan particles (CP-PEI-Zn) were prepared by cross-linking chitosan particles (CP) with sodium tripolyphosphate (TPP), modifying with metal chelator polyethylenimine (PEI), and subsequent chelating of Zn2+. The coordination interaction was confirmed by analyzing the adsorption and desorption behavior of iFMDV on CP-PEI-Zn by high-performance size exclusion chromatography (HPSEC), while the CP-PEI without chelating Zn2+ loads iFMDV mainly through electrostatic interactions. The iFMDV loaded on CP-PEI-Zn showed better thermal stability than that on CP-PEI, as revealed by a slightly higher transition temperature (Tm) related to iFMDV dissociation. After subcutaneous immunization in female Balb/C mice, antigens loaded on CP-PEI and CP-PEI-Zn all induced higher specific antibody titers, better ...Continue Reading

References

Apr 1, 1992·Journal of Virology·K C McCulloughU Kihm
Nov 28, 1991·Nature·R C LiddingtonS C Harrison
Mar 26, 1999·The Journal of General Virology·A J ChilderstoneR M Parkhouse
Mar 22, 2003·Journal of Chromatography. a·E K M UedaL Morganti
Sep 25, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gautam BasuTrevor Douglas
Feb 3, 2005·The Journal of Biological Chemistry·LaToya S JonesC Russell Middaugh
Feb 2, 2006·Biomaterials·Tadashi Kokubo, Hiroaki Takadama
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Sue D XiangMagdalena Plebanski
Aug 30, 2008·Blood·Jinfang Zhu, William E Paul
Feb 26, 2009·Expert Opinion on Drug Delivery·Christopher F van der WalleMnv Ravi Kumar
Jan 19, 2010·Current Opinion in Microbiology·Allison C Rice-FichtThomas A Ficht
Oct 16, 2010·Nature Reviews. Immunology·Martin F Bachmann, Gary T Jennings
May 15, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Medha D JoshiEnrico Mastrobattista
Aug 28, 2012·Nature Biotechnology·Frank WegmannQuentin J Sattentau
Oct 5, 2012·The Journal of General Virology·B Veronica CarrBryan Charleston
Dec 4, 2013·Vaccine·Liang ZhaoAnton P J Middelberg
Dec 23, 2014·Nanomedicine·Ruchi R ShahLuis A Brito
Jan 13, 2015·Colloids and Surfaces. B, Biointerfaces·Yiguang JinLina Du
Nov 23, 2016·Journal of Immunology Research·Shintaro Hojyo, Toshiyuki Fukada
May 24, 2017·Nature Communications·Abhay KotechaDavid I Stuart
Jun 2, 2018·Biomolecular Concepts·Gunjan KakBrijendra K Tiwari
Feb 5, 2019·Environmental Science and Pollution Research International·Azza HafezElsayed Hegazi
Jun 14, 2019·Heliyon·Nazrul IslamMutasem O Taha
Dec 21, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Leticia Santos PimentelPaula Cristina Batista de Faria
Feb 23, 2020·International Journal of Biological Macromolecules·Nabel A NegmMaram T H Abou Kana
Mar 8, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yanli YangZhiguo Su
Feb 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jia-Wei LiuJian-Hua Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.