A novel 'peak parking' strategy for ultra-performance liquid chromatography/tandem mass spectrometric detection for enhanced performance of bioanalytical assays

Rapid Communications in Mass Spectrometry : RCM
Fumin LiQin C Ji

Abstract

Sensitive and high-throughput bioanalytical assays are of vital importance to drug discovery and development. Ultra-performance liquid chromatography (UPLC), utilizing sub-2-microm particles, greatly increases the separation throughput and efficiency, resulting in LC peaks as narrow as or less than 1 s (full width at half maxima, FWHM). This, however, could pose practical challenges for bioanalytical applications using quadrupole mass spectrometry (MS) to acquire sufficient data points to ensure accurate and reliable quantitation. Here, we present a novel 'peak parking' strategy to reduce the flow rate during UPLC peak elution, therefore extending the useful MS acquisition window. The high-throughput advantage of UPLC is maintained since no significant increase of the overall UPLC run time is needed. This strategy was demonstrated in an assay development for lansoprazole, a gastric proton-pump inhibitor, in human plasma employing liquid-liquid extraction. The method was validated from 50.0 to 50,000 pg/mL.

References

Jun 9, 1995·Journal of Chromatography. B, Biomedical Applications·M D KarolK Alexander
Mar 22, 2000·Journal of Pharmaceutical and Biomedical Analysis·A A Moustafa
Dec 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Celso H OliveiraGilberto de Nucci
Sep 24, 2004·Rapid Communications in Mass Spectrometry : RCM·Robert PlumbAndrew Wright
Jul 9, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mona I ChurchwellDaniel R Doerge
Jun 22, 2006·Current Drug Metabolism·Mohammed Jemal, Yuan-Qing Xia

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Citations

Jun 25, 2009·Analytical Chemistry·Stephen J Hattan, Marvin L Vestal
Jul 4, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qin-You TanWen-Xing Peng
Aug 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yanwen LiuYanqing Lei
Apr 14, 2011·Rapid Communications in Mass Spectrometry : RCM·Andrew D WagnerWilson Z Shou
Nov 19, 2014·Journal of Analytical Toxicology·Muzaffar IqbalAmal A Bajrai
Jan 27, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Muzaffar IqbalFaiyaz Shakeel
Nov 17, 2015·Journal of Analytical Toxicology·Amal A BajraiMuzaffar Iqbal

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