A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3

Vaccines
Ruihua ZhangShijin Jiang

Abstract

Duck hepatitis A virus (DHAV), the major pathogen of duck virus hepatitis (DVH), causes severe diseases that threaten the duck industry worldwide. The VP1 protein, a major structural protein of DHAV, is able to induce neutralizing antibody in ducks. The purpose of this study was to identify the antigenic mimotope of DHAV by phage display technology. A monoclonal antibody (mAb) 4E6 against DHAV-1 and DHAV-3 was prepared, and a phage library prepared with the PhD-12 Phage Display Peptide Library Kit was screened with the mAb. A novel peptide, 1GLTWKLPPSM10 was identified with high affinity to the mAb and could specifically block mAb 4E6 from binding DHAV-1 and DHAV-3. Animal tests confirmed that the immunization of ducklings with the mimotope could inhibit the virus proliferation and protect the ducklings from DVH. In summary, the neutralizing conformational mimotope 1GLTWKLPPSM10 might be a promising vaccine candidate for the prevention of DHAV infection.

References

Nov 13, 2004·The Journal of Biological Chemistry·Beihai JiangChengchao Shou
Feb 13, 2007·Virus Research·Chun-Hsien Tseng, Hsiang-Jung Tsai
Feb 16, 2007·Virology·Chunyu Ding, Dabing Zhang
Nov 2, 2014·Archives of Virology·Mahmoud SolimanMun-Il Kang
Jul 10, 2018·Veterinary Microbiology·Ruihua ZhangShijin Jiang

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Methods Mentioned

BETA
phage display
Assay
Transfection
electrophoresis
enzyme linked immunosorbent assay
ELISA
antisense oligonucleotide

Software Mentioned

DNASTAR
Lasergene
GraphPad

Related Concepts

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