A novel peripherin isoform generated by alternative translation is required for normal filament network formation

Journal of Neurochemistry
Jesse McLeanJanice Robertson

Abstract

Peripherin is a type III neuronal intermediate filament protein detected within the intraneuronal inclusions characteristic of amyotrophic lateral sclerosis. The constitutively expressed peripherin isoform is encoded by all nine exons of the human and mouse peripherin genes to generate a protein species of approximately 58 kDa on sodium dodecyl sulfate-polyacrylamide gels. Expression of this isoform, termed Per-58, generates a filament network in transfected SW13 vim cells. On immunoblots of cell lysates derived from these transfected cells, we have consistently observed a second peripherin species of approximately 45 kDa. In this study, we show that this species is a novel peripherin isoform generated through the use of an in-frame downstream initiation codon. This isoform, that we have designated Per-45, is co-expressed together with Per-58 and, thus, constitutive in both human and mouse. Using mutational analysis, we show that Per-45 is required for normal network formation, with the absence of Per-45 leading to irregular filamentous structures. We further show that peripherin expression in the normal nervous system is characterized by tissue-specific Per-58 : Per-45 isoform ratios. Taken together, these results identify nov...Continue Reading

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Citations

Jan 16, 2013·The Journal of Biological Chemistry·Peng-Han SuHsi-Yuan Yang
Jan 12, 2010·Journal of Autoimmunity·Jayne L ChamberlainVanda A Lennon
Dec 3, 2017·Cold Spring Harbor Perspectives in Biology·Elly M Hol, Yassemi Capetanaki
Apr 20, 2011·Journal of Cell Science·Claire L HyderJohn E Eriksson

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