A novel phosphoglucose isomerase (PGI)/phosphomannose isomerase from the crenarchaeon Pyrobaculum aerophilum is a member of the PGI superfamily: structural evidence at 1.16-A resolution

The Journal of Biological Chemistry
Michael K SwanChristopher Davies

Abstract

The crystal structure of a dual specificity phosphoglucose isomerase (PGI)/phosphomannose isomerase from Pyrobaculum aerophilum (PaPGI/PMI) has been determined in native form at 1.16-A resolution and in complex with the enzyme inhibitor 5-phosphoarabinonate at 1.45-A resolution. The similarity of its fold, with the inner core structure of PGIs from eubacterial and eukaryotic sources, confirms this enzyme as a member of the PGI superfamily. The almost total conservation of amino acids in the active site, including the glutamate base catalyst, shows that PaPGI/PMI uses the same catalytic mechanisms for both ring opening and isomerization for the interconversion of glucose 6-phosphate (Glc-6-P) to fructose 6-phosphate (Fru-6-P). The lack of structural differences between native and inhibitor-bound enzymes suggests this activity occurs without any of the conformational changes that are the hallmark of the well characterized PGI family. The lack of a suitable second base in the active site of PaPGI/PMI argues against a PMI mechanism involving a trans-enediol intermediate. Instead, PMI activity may be the result of additional space in the active site imparted by a threonine, in place of a glutamine in other PGI enzymes, which could p...Continue Reading

References

Sep 1, 1993·Applied and Environmental Microbiology·P VölklK O Stetter
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S H Seeholzer
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y J SunC D Hsiao
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·J W Pflugrath
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Diana ArsenievaConstance J Jeffery
May 31, 2002·Acta Crystallographica. Section D, Biological Crystallography·Richard J MorrisVictor S Lamzin
Oct 9, 2002·Journal of Molecular Biology·Diana Arsenieva, Constance J Jeffery
Feb 22, 2003·Acta Crystallographica. Section D, Biological Crystallography·Christopher Davies, Hilary Muirhead
Jun 5, 2003·Acta Crystallographica. Section D, Biological Crystallography·Christopher DaviesJohn Chirgwin
Jun 11, 2003·The Journal of Biological Chemistry·John M BerrisfordPatrick J Baker
Sep 13, 2003·The Journal of Biological Chemistry·Michael K SwanChristopher Davies
Oct 24, 2003·Acta Crystallographica. Section D, Biological Crystallography·G BricogneW Paciorek
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Michael K SwanChristopher Davies
Jan 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·J P Abrahams, A G Leslie
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Feb 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Giri GowdaM R N Murthy
Mar 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ken-ichi AokiKazuo T Nakamura
May 7, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kanchan AnandLalit C Garg
Oct 31, 2014·Proteins·Maayan Bonjack, David Avnir
Nov 1, 2005·Current Opinion in Microbiology·Bettina Siebers, Peter Schönheit
Feb 29, 2016·Applied Biochemistry and Biotechnology·Manisha J PatelDarshan H Patel
Jun 16, 2012·Biology of Reproduction·Deivendran RengarajJae Yong Han
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Christopher BräsenBettina Siebers
Feb 16, 2006·Proteins·Jayaraman SeetharamanSubramanyam Swaminathan
May 8, 2019·Acta Crystallographica. Section D, Structural Biology·Mamata BangeraM R N Murthy

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