A Novel PhosphorImager-Based Technique for Monitoring the Microbial Reduction of Technetium.

Applied and Environmental Microbiology
J R Lloyd, L E Macaskie

Abstract

A novel PhosphorImager-based technique which can be used to quantify low concentrations of radionuclides is described. The technique offers several benefits, combining very high sensitivity with containment of the radioisotope in the solid state, thus minimizing disposal procedures. In this study, it was used in conjunction with paper chromatography to quantify different oxidation states of (sup99)Tc in solution. The technique was used to evaluate the potential of anaerobic cultures of Shewanella putrefaciens and Geobacter metallireducens (bacteria with known metal-reducing capabilities) to reduce highly soluble Tc(VII) to insoluble lower-valence species, facilitating its removal from solution. Both organisms reduced Tc(VII), but profiles of Tc species produced in culture supernatants were strain specific. S. putrefaciens produced Tc(V), Tc(IV), and one unidentified species, but no Tc was removed from solution. G. metallireducens removed 70% of the 250 (mu)M Tc added in solution, with trace amounts of Tc(V) and the unidentified species detected in culture supernatants. Possible uses for these organisms in the bioremediation of Tc-contaminated waters are discussed, and other uses of the PhosphorImager technique are highlighted.

References

Feb 1, 1992·Applied and Environmental Microbiology·K H Nealson, C R Myers
May 1, 1990·Electrophoresis·R F JohnstonD L Barker
Nov 1, 1989·Health Physics·L PignoletZ Moureau
Aug 1, 1989·Health Physics·J P DehutC M Vandecasteele
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 1, 1993·Annual Review of Microbiology·D R Lovley
Aug 1, 1986·Plant Physiology·J F Lembrechts, G M Desmet
Jun 1, 1993·Trends in Ecology & Evolution·D R Lovley

❮ Previous
Next ❯

Citations

Sep 26, 2001·Applied and Environmental Microbiology·G De LucaA Verméglio
Aug 9, 2003·Letters in Applied Microbiology·C R Myers, J M Myers
Apr 27, 2013·Microbial Biotechnology·Dhan PrakashOm V Singh
Oct 16, 2002·Biotechnology and Bioengineering·Chongxuan LiuChristopher F Brown
Feb 7, 2004·Biotechnology Progress·Sridhar ViamajalaJames N Petersen
Oct 31, 1998·Applied and Environmental Microbiology·J R LloydL E Macaskie
Jul 31, 2003·Biotechnology and Bioengineering·Sridhar ViamajalaJames N Petersen
Mar 4, 2000·Applied and Environmental Microbiology·K Kashefi, D R Lovley
Jul 10, 2007·Applied Microbiology and Biotechnology·Nikolay A ChernyhAlexander I Slobodkin
Jul 31, 2003·Biotechnology and Bioengineering·Sarah S MiddletonCraig S Criddle
May 1, 2003·FEMS Microbiology Ecology·Tatiana V KhijniakMonique Simonoff
Oct 24, 2006·Applied and Environmental Microbiology·Ken Fujimoto, Takami Morita
Nov 26, 2009·Critical Reviews in Biotechnology·Hassan KorbekandiSajjad Abbasi
Nov 8, 2012·Applied Microbiology and Biotechnology·Guangfei LiuHong Lv
Dec 4, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Andrea CherkoukJonathan R Lloyd
May 15, 2015·Journal of the Royal Society, Interface·J M ByrneJ R Lloyd
Aug 28, 2007·Bioinformatics·Raman Lall, Julie Mitchell
Nov 12, 2005·Archives of Microbiology·Oleg A ZadvornyIvan N Gogotov
Nov 5, 2005·Applied and Environmental Microbiology·Rizlan Bencheikh-LatmaniBradley M Tebo
Aug 31, 2000·Applied and Environmental Microbiology·J R LloydD R Lovley
May 2, 2000·Applied and Environmental Microbiology·J K FredricksonM J Daly
Aug 9, 2020·Journal of Hazardous Materials·Mahmoud NasrollahzadehRajender S Varma
Mar 1, 1997·Journal of Bacteriology·J R LloydL E Macaskie
Dec 14, 2004·The Science of the Total Environment·A AbdelouasE Leclerc-Cessac
Sep 10, 2014·Environmental Science & Technology·Ashley R BrownJonathan R Lloyd
Jan 8, 2011·Environmental Science & Technology·Andrew E PlymalePonnusamy Nachimuthu
Nov 17, 2017·Environmental Science & Technology·Nicholas K Masters-WaageGareth T W Law
Oct 16, 2015·Environmental Science & Technology·Yuanyuan LiuCharles T Resch
Apr 16, 2008·Environmental Science & Technology·Xiangzhen Li, Lee R Krumholz
Jun 30, 2005·Environmental Science & Technology·Ian T BurkeKatherine Morris

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.