Abstract
A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 degrees C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO2 evolved in the test and control calculated gravimetrically through Sturm test indicated the degradative capabilities of Streptoverticillium kashmirense AF1.
References
Jan 1, 1995·Canadian Journal of Microbiology·J MergaertJ Swings
Sep 1, 1993·Journal of Biomedical Materials Research·S GogolewskiM K Hughes
Oct 1, 1993·Applied and Environmental Microbiology·J MergaertJ Swings
Nov 1, 1993·Thorax·P G Holt
Aug 1, 1993·International Journal of Biological Macromolecules·K YamadaY Doi
Apr 1, 1993·Applied and Environmental Microbiology·A SchirmerH G Schlegel
Dec 1, 1995·Chemosphere·U PaggaB De Wilde
Jul 1, 1996·International Journal of Systematic Bacteriology·J MergaertJ Swings
Nov 26, 1997·Journal of Bacteriology·M Nojiri, T Saito
Jan 30, 1999·Applied and Environmental Microbiology·S H ImamR V Greene
Dec 2, 2000·Chemosphere·U PaggaM Pantke
Mar 14, 2001·Journal of Biotechnology·D M Abou-ZeidW D Deckwer
Jun 21, 2001·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·M N KimJ S Yoon
Aug 7, 2001·Biodegradation·F J CervantesJ A Field
Aug 7, 2001·Biodegradation·A Manna, A K Paul
Jul 9, 2002·Biomacromolecules·Simone ReinhardtDieter Jendrossek
Aug 4, 2004·Biotechnology Letters·Yutaka Tokiwa, Buenaventurada P Calabia
Sep 2, 2004·Archives of Microbiology·Fabian RomenDieter Jendrossek
Aug 19, 2005·The Journal of General and Applied Microbiology·Nevine B GhanemDalia E S El-Badan
Apr 15, 2006·Biotechnology Letters·Buenaventurada P Calabia, Yutaka Tokiwa