PMID: 8939024Nov 1, 1996Paper

A novel polymerase chain reaction assay for the detection and speciation of thermophilic Campylobacter spp

The Journal of Applied Bacteriology
C J JacksonD M Jones

Abstract

A novel PCR amplification method is described which is specific for the thermophilic, enteropathogenic species Campylobacter jejuni, Camp. coli and Camp. upsaliensis. Rapid, accurate speciation of amplified strains is possible on the basis of restriction fragment length polymorphisms of PCR products digested with three restriction enzymes, AluI, DdeI and DraI. The sensitivity of detection is 25 cfu in water, and 2 x 10(3) cfu in full cream milk. An epidemiological application of the assay in detecting non-culturable campylobacters from a contaminated potable water supply is described.

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Citations

Oct 3, 2003·Diagnostic Microbiology and Infectious Disease·Gerardo ManfredaAchille Franchini
Nov 19, 2002·FEMS Microbiology Letters·Tracey A BurnettSteven P Djordjevic

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