A novel potentiometric immunoassay for carcinoma antigen 15-3 by coupling enzymatic biocatalytic precipitation with a nanogold labelling strategy

The Analyst
Kun DengXue-Dong Tong

Abstract

Methods based on potentiometric measurement have been developed for immunoassays, but most exhibit low sensitivities and are unsuitable for early diagnosis of disease. Herein we design a new potentiometric immunosensing platform for the sensitive detection of carcinoma antigen 15-3 (CA 15-3) by coupling with enzymatic biocatalytic precipitation and a nanogold labeling technique. The sandwich-type immunoreaction is carried out on a monoclonal anti-CA 15-3 capture antibody-modified working electrode, using horseradish peroxidase (HRP) and polyclonal anti-CA 15-3 detection antibody-labeled gold nanoparticles. Upon the introduction of target CA 15-3, the carried HRP molecules with an immunocomplex catalyze the oxidation of 4-chloro-1-naphthol (4-CN) into the insoluble benzo-4-chlorohexadienone. The formed product coated on the surface of the modified electrode results in a change of the electrical potential. Under optimal conditions, the shift in the electrical potential relative to the background signal increases with the increasing target CA 15-3 concentration, and exhibits a good linear relationship within the dynamic range of 0.01-30 U mL-1 at a detection limit of 7.8 mU mL-1. Good precision and reproducibility, and high specif...Continue Reading

References

Feb 28, 2016·Analytica Chimica Acta·Xu WangDietmar Knopp
Jul 30, 2016·Biosensors & Bioelectronics·Pei-Xin YuanDan Shan
Sep 8, 2017·Chemistry, an Asian Journal·Jian Shu, Dianping Tang
Oct 27, 2017·The Breast : Official Journal of the European Society of Mastology·Diana LüftnerGuenther G Steger

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Citations

Nov 13, 2021·Angewandte Chemie·Sumit PalDibyendu Das

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Methods Mentioned

BETA
ELISA
transmission electron microscopy
dynamic light scattering

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