A novel pro-inflammatory mechanism of action of resistin in human endothelial cells: up-regulation of SOCS3 expression through STAT3 activation

Biochemical and Biophysical Research Communications
Monica PirvulescuMaya Simionescu

Abstract

Resistin is a significant local and systemic regulatory cytokine involved in inflammation. Suppressors of cytokine signaling (SOCS) proteins are intracellular regulators of receptor signal transduction induced by several cytokines in a cytokine and cell specific manner. Resistin up-regulates SOCS3 expression in mice adipocytes but it is not known whether this is a common occurrence in other cells. We questioned whether resistin-induces SOCS3 in human endothelial cells and if signal transducer and activator of transcription (STAT) proteins are involved in the process. The Real-Time PCR and Western blot analysis showed that in resistin-activated HEC the gene and protein expression of SOCS3 were significantly increased. Furthermore, resistin induced activation of STAT3 as characterized by increased tyrosine phosphorylation. Resistin-induced SOCS3 expression was blocked by specific inhibitors of STAT3 signaling and by the transfection of siRNA specific for STAT3. Silencing of SOCS3 gene expression by transfection with SOCS3 siRNA reduced the expression of resistin induced-P-selectin and fractalkine in HEC. Together, our results demonstrate that in HEC (1) resistin up-regulates SOCS3 expression and activates STAT3 transcription fact...Continue Reading

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Dec 26, 2009·Biochemical and Biophysical Research Communications·Ileana ManduteanuMaya Simionescu

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Citations

Aug 19, 2014·World Journal of Gastroenterology : WJG·Kamila WójcikAnna Piekarska
Sep 23, 2014·General and Comparative Endocrinology·Ajit SinghAmitabh Krishna
May 27, 2014·Expert Opinion on Drug Discovery·Morena ScoteceOreste Gualillo
Feb 24, 2016·Pediatric Hematology and Oncology·Szymon SkoczenMaciej Siedlar
Feb 20, 2014·Biochemical and Biophysical Research Communications·Fengyun WenZaiqing Yang
Aug 3, 2013·Drug Discovery Today·Morena ScoteceOreste Gualillo
May 13, 2015·Biochemical Genetics·Fengyun WenZaiqing Yang
Feb 16, 2015·Molecular Biology Reports·Chandra S Boosani, Devendra K Agrawal
Jan 8, 2014·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Wenbing QiuHong Jin
Jul 16, 2016·Experimental & Molecular Medicine·Sang-Min Jeon
Jan 15, 2014·Molecular Biology Reports·Xiaotao FengQiuyan Jiang
Apr 11, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shaoning JiangJaroslaw W Zmijewski
Jul 6, 2021·Frontiers in Immunology·Yanran LiZhicai Zuo
Sep 1, 2021·Clinical and Experimental Medicine·Chiara PellicanoEdoardo Rosato
Aug 18, 2020·The American Journal of Pathology·Chiara MacchiMassimiliano Ruscica

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