Nov 9, 2011

A novel property of propolis (bee glue): anti-pathogenic activity by inhibition of N-acyl-homoserine lactone mediated signaling in bacteria

Journal of Ethnopharmacology
Zackery BulmanMichael A Savka

Abstract

An alternative approach to antibiotics is the development of anti-pathogenic agents to control the bacterial virulome. Such anti-pathogenic agents could target a phenomena known as quorum sensing (QS). Six bacterial N-acyl-homoserine lactone (AHL)-dependent bioreporter strains were used to evaluate if bee hive glue also known as propolis contains constituents capable of inhibiting QS-controlled AHL signaling. In addition, the effect of propolis on the QS-dependent swarming motility was evaluated with the opportunisitic pathogen, Pseudomonas aeruginosa. Differences in the propolis tincture samples were identified by physiochemical profiles and absorption spectra. Propolis tinctures at 0.0005% (v/v) that do not affect bacteria biosensor growth or the reporter system monitored were exposed to biosensors with and without the addition an AHL. No AHL signal mimics were found to be present in the propolis tinctures. However, when propolis and an inducer AHL signal were together exposed to five Escherichia coli and a Chromobacterium violaceum biosensor, propolis disrupted the QS bacterial signaling system in liquid- and agar-based bioassays and in C(18) reverse-phase thin-layer plate assays. Swarming motility in the opportunistic patho...Continue Reading

  • References47
  • Citations6

References

  • References47
  • Citations6

Mentioned in this Paper

Chromobacterium
Bacterial Proteins
Pseudomonas aeruginosa (antigen)
Alkalescens-Dispar Group
Cell Motility
Antibiotic throat preparations
Agar
Pathogenic Organism
Biosensors
Antifungal Antibiotics, Topical

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