A novel protein expression strategy using recombinant bovine respiratory syncytial virus (BRSV): modifications of the peptide sequence between the two furin cleavage sites of the BRSV fusion protein yield secreted proteins, but affect processing and function of the BRSV fusion protein

The Journal of General Virology
Patricia KönigG M Keil

Abstract

The bovine respiratory syncytial virus (BRSV) fusion (F) protein is cleaved at two furin cleavage sites, which results in generation of the disulfide-linked F(1) and F(2) subunits and release of an intervening peptide of 27 aa (pep27). A series of mutated open reading frames encoding F proteins that lacked the entire pep27, that contained an arbitrarily chosen 23 aa sequence instead of pep27 or in which pep27 was replaced by the amino acid sequences for the bovine cytokines interleukin 2 (boIL2), interleukin 4 (boIL4) or gamma interferon (boIFN-gamma) was constructed. Transient expression experiments revealed that the sequence of the intervening peptide influenced intracellular transport, maturation of the F protein and F-mediated syncytium formation. Expression of boIL2, boIL4 or boIFN-gamma in place of pep27 resulted in secretion of the cytokines into the culture medium. All mutated F proteins except the boIFN-gamma-containing variant could be expressed by and were functional for recombinant BRSV. Characterization of the cell culture properties of the recombinants demonstrated that the amino acid sequence between the two furin cleavage sites affected entry into target cells, direct spreading of virions from cell to cell and v...Continue Reading

References

Jan 1, 1990·Archives of Virology·S K MallipeddiS B Mohanty
Feb 1, 1994·Trends in Microbiology·H D Klenk, W Garten
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·A BukreyevP L Collins
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·L González-ReyesJ A Melero
Oct 13, 2001·The New England Journal of Medicine·J M Shapiro, R E Jean

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Citations

Jan 25, 2008·Journal of Virology·Julia Eckardt-MichelGert Zimmer
Mar 3, 2010·Veterinary Microbiology·Günther M KeilJutta Veits
Jan 30, 2007·Veterinary Research·Jean-Francois Valarcher, Geraldine Taylor
Aug 17, 2020·Journal of Virology·Zhaolong LiWenyan Zhang

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