A novel protein modification pathway related to the ubiquitin system

The EMBO Journal
D LiakopoulosS Jentsch

Abstract

Ubiquitin conjugation is known to target protein substrates primarily to degradation by the proteasome or via the endocytic route. Here we describe a novel protein modification pathway in yeast which mediates the conjugation of RUB1, a ubiquitin-like protein displaying 53% amino acid identity to ubiquitin. We show that RUB1 conjugation requires at least three proteins in vivo. ULA1 and UBA3 are related to the N- and C-terminal domains of the E1 ubiquitin-activating enzyme, respectively, and together fulfil E1-like functions for RUB1 activation. RUB1 conjugation also requires UBC12, a protein related to E2 ubiquitin-conjugating enzymes, which functions analogously to E2 enzymes in RUB1-protein conjugate formation. Conjugation of RUB1 is not essential for normal cell growth and appears to be selective for a small set of substrates. Remarkably, CDC53/cullin, a common subunit of the multifunctional SCF ubiquitin ligase, was found to be a major substrate for RUB1 conjugation. This suggests that the RUB1 conjugation pathway is functionally affiliated to the ubiquitin-proteasome system and may play a regulatory role.

References

Jan 1, 1992·Annual Review of Genetics·S Jentsch
Jun 30, 1992·Biochemical and Biophysical Research Communications·S KumarM Noda
May 1, 1990·Trends in Biochemical Sciences·S JentschH A Reins
Apr 5, 1987·Journal of Molecular Biology·S Vijay-KumarW J Cook
Jul 28, 1995·The Journal of Biological Chemistry·R J DohmenA Varshavsky
Oct 7, 1994·Cell·A Ciechanover
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Aug 15, 1997·The EMBO Journal·S TakayamaJ C Reed
Oct 6, 1997·The EMBO Journal·J M Galan, R Haguenauer-Tsapis
Oct 27, 1997·The Journal of Biological Chemistry·E S Johnson, G Blobel
Nov 14, 1997·The Journal of Biological Chemistry·T KamitaniE T Yeh
Nov 14, 1997·Trends in Biochemical Sciences·H SaitohM Dasso
Nov 14, 1997·Trends in Biochemical Sciences·A Varshavsky
Mar 21, 1998·Current Biology : CB·H SaitohM Dasso
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S E SchwarzS Jentsch

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Citations

Mar 10, 2000·International Journal of Cancer. Journal International Du Cancer·P M McLaughlinC H Buys
Jan 10, 2012·Inflammatory Bowel Diseases·Stefan F Ehrentraut, Sean P Colgan
Jan 9, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sean P ColganStefan F Ehrentraut
Feb 17, 2006·Molecular Genetics and Genomics : MGG·S PöggelerU Kück
Dec 3, 1999·Progress in Biophysics and Molecular Biology·K L Craig, M Tyers
May 12, 2000·Gene·E T YehT Kamitani
Jul 8, 2000·Trends in Cell Biology·S Jentsch, G Pyrowolakis
Mar 1, 2000·Trends in Biochemical Sciences·W M Gray, I Estelle
May 14, 1999·Trends in Plant Science· del Pozo JC, M Estelle
Oct 6, 2000·Current Opinion in Plant Biology·J Callis, R D Vierstra
Mar 6, 1999·Current Opinion in Plant Biology·L Walker, M Estelle
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Matthew D Petroski, Raymond J Deshaies
Apr 9, 2009·Nature Reviews. Molecular Cell Biology·Brenda A Schulman, J Wade Harper
Feb 12, 2008·Nature Structural & Molecular Biology·Danny T HuangBrenda A Schulman
Sep 14, 2004·Nature Structural & Molecular Biology·Danny T HuangBrenda A Schulman
Jan 21, 2011·The Journal of Biological Chemistry·Guochang HuangBhuvanesh Singh
Nov 14, 2006·The Journal of Biological Chemistry·Wassim M AbidaWei Gu
May 8, 1999·Molecular Biology of the Cell·I TanidaE Kominami
Oct 11, 2003·Molecular Biology of the Cell·April S GoehringGeorge F Sprague
Apr 9, 2011·Carcinogenesis·Keita NoguchiShigetsugu Hatakeyama
Dec 19, 1998·The EMBO Journal·A Ciechanover
Aug 3, 2001·The EMBO Journal·T KawakamiK Tanaka
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A R WillemsM Tyers
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R T HayM S Rodriguez
Aug 8, 1998·Genes & Development·F OsakaS Kato
Sep 29, 1999·Genes & Development·J D SingerJ M Roberts
Jul 1, 1999·Protein Science : a Publication of the Protein Society·A M WyndhamG Chelvanayagam
Aug 12, 2004·Science's STKE : Signal Transduction Knowledge Environment·Olivier Staub
Mar 17, 2009·Eukaryotic Cell·Wilfried Jonkers, Martijn Rep
Dec 14, 2011·Eukaryotic Cell·Michelle D Leach, Alistair J P Brown

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