A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer

Microbial Cell Factories
Nehal M El-DeebAmr El-Hawiet

Abstract

The direct link between inflammatory bowel diseases and colorectal cancer is well documented. Previous studies have reported that some lactic acid bacterial strains could inhibit colon cancer progression however; the exact molecules involved have not yet been identified. So, in the current study, we illustrated the tumor suppressive effects of the newly identified Lactobacillus acidophilus DSMZ 20079 cell-free pentasaccharide against colon cancer cells. The chemical structure of the purified pentasaccharide was investigated by MALDI-TOF mass spectrum, 1D and 2D Nuclear Magnetic Resonance (NMR). The anticancer potentiality of the purified pentasaccharide against both Human colon cancer (CaCo-2) and Human breast cancer (MCF7) cell lines with its safety usage pattern were evaluated using cytotoxicity, annexin V quantification and BrdU incorporation assays. Also, the immunomodulatory effects of the identified compound were quantified on both LPS-induced PBMC cell model and cancer cells with monitoring the immunophenotyping of T and dendritic cell surface marker. At molecular level, the alteration in gene expression of both inflammatory and apoptotic pathways were quantified upon pentasaccharide-cellular treatment by RTqPCR. The obt...Continue Reading

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Citations

Mar 17, 2020·Medicine·Vorraaud KunnavuttivanichSuthinee Ithimakin
Oct 27, 2018·Frontiers in Microbiology·Nuria Castro-BravoPatricia Ruas-Madiedo
Nov 9, 2020·Microbial Cell Factories·Wallace Jeng Yang CheeLeslie Thian Lung Than
Apr 20, 2021·Heliyon·Ahmed A EmamAhmed M A El-Sokkary
Jun 3, 2021·Microorganisms·Edgar Torres-MaravillaLuis G Bermúdez-Humarán
Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tesfaye Legesse BedadaDagim Jirata Birri
Nov 9, 2021·Critical Reviews in Food Science and Nutrition·Harsh KumarNatália Cruz-Martins

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Methods Mentioned

BETA
NMR
2D-NMR
ELISA
flow cytometry
PCR
nuclear translocation

Software Mentioned

graphpad prism
Flex Analysis
CASPER
CFX Manager

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