A Novel Quinolyl-Substituted Analogue of Resveratrol Inhibits LPS-Induced Inflammatory Responses in Microglial Cells by Blocking the NF-κB/MAPK Signaling Pathways

Molecular Nutrition & Food Research
Yue HouChun Fu Wu

Abstract

The anti-neuroinflammatory effect of a novel quinolyl-substituted analogue of resveratrol (RV01) on lipopolysaccharide (LPS)-induced microglial activation is investigated, as well as the possible underlying mechanisms. Cell viability is measured using an MTT assay. Nitric oxide (NO) release is determined by nitrite assay. The interaction between RV01 and inducible nitric oxide synthase (iNOS) is studied using molecular docking. Free radical scavenging activity and reactive oxygen species (ROS) production are determined by DPPH reduction assay and DCFH-DA assay. Pretreatment with RV01 (1-30 µm) prior to LPS (1 µg mL-1 ) stimulation decreased NO release and iNOS expression without observable cytotoxicity. RV01 reduced the mRNA levels and secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). RV01 also inhibited LPS-induced ROS production and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation. Furthermore, RV01 decreases the protein expression of toll-like receptor 4 (TLR4) and inhibits the LPS-induced activation of the mitogen-activated protein kinase (MAPK) and nuclear transcription factor-κB (NF-κB) signaling pathways. Additionally, conditioned medium from microglia co-treated with LPS and RV...Continue Reading

References

Jan 5, 2002·Neurobiology of Aging·R T PerryR C Go
Jun 2, 2009·Bioorganic & Medicinal Chemistry·Brian W MoranPeter T M Kenny
Nov 24, 2011·Molecular Aspects of Medicine·Jeremy P E SpencerDavid Vauzour
Nov 12, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiaoxiao WangJingyu Yang
Jun 3, 2014·International Journal of Endocrinology·Reddemma SandireddyAshutosh Kumar
Jun 19, 2014·Current Pharmaceutical Biotechnology·Justine Renaud, Maria-Grazia Martinoli
Jan 20, 2015·Molecular Neurobiology·Yu Tang, Weidong Le
Jun 13, 2015·Brain, Behavior, and Immunity·Yan LiPatrick M Dougherty
Sep 30, 2015·Current Pharmaceutical Design·Chiara PorroMaria Antonietta Panaro
Nov 12, 2015·Biochemical Pharmacology·Mithilesh Kumar JhaKyoungho Suk
Feb 27, 2017·Ageing Research Reviews·Mahban RahimifardSeyed Fazel Nabavi
Sep 25, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shan HuangPing Lei
Nov 8, 2017·Nutrients·Waqas NawazXiaohong Shu
Nov 8, 2017·Oxidative Medicine and Cellular Longevity·Adam RibaRobert Halmosi
Jan 11, 2018·Cell Death & Disease·Xiaoyu WangXuechu Zhen
Jan 18, 2018·Molecular Nutrition & Food Research·Sarah Tomas-HernándezMiquel Mulero
Jan 30, 2018·Frontiers in Molecular Neuroscience·Guo-Qing WangFeng Zhang
Mar 3, 2018·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ru ChenNing Li
Mar 9, 2018·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Amynah A PradhanSimon Akerman
Apr 4, 2018·Proceedings of the National Academy of Sciences of the United States of America·Shuliang ChenLi Wu
Apr 21, 2018·Frontiers in Molecular Neuroscience·Davide PozziMichela Matteoli
Apr 28, 2018·Cytokine & Growth Factor Reviews·Nese Unver, Florencia McAllister

❮ Previous
Next ❯

Citations

Mar 25, 2020·Phytotherapy Research : PTR·Wenqiang ZhangYue Hou
Dec 25, 2019·Metallomics : Integrated Biometal Science·Yifei HeYonggui He
Nov 22, 2020·The Journal of Nutritional Biochemistry·Juan HuangFeng Jin
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tiantian MengJianhua He

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.