A novel rapid method for simultaneous determination of eight active compounds in silymarin using a reversed-phase UPLC-UV detector

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Hong LiuQipeng Yuan

Abstract

A novel rapid chromatographic method based on utilization of UPLC column was developed for the analysis of eight active compounds in silymarin. The analysis was performed on a Waters Acquity UPLC system with an Acquity UPLC(BEH) C18 column (5mmx2.1mm I.D., 1.7mum) and a gradient elution of methanol and water containing 0.01% formic acid with a run time of 9min, in which the retention time of the last analyte was 5.8min. And all eight active compounds achieved complete separation. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The results indicated that the type of column, the type of mobile phase and the modified addition were significant to the separation of isomeric compounds in herb extracts.

References

Sep 5, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chandrani Gunaratna, Tianyi Zhang
Mar 28, 2006·Journal of Chromatography. a·Stephen A C Wren, Pierre Tchelitcheff
Sep 8, 2006·Fitoterapia·Frantisek SersenJulius Annus
Nov 11, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jurgen MenschMarcus E Brewster
Oct 31, 2008·Talanta·Lucie NovákováPetr Solich

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Citations

Jun 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Dezső CsuporJudit Hohmann
Feb 23, 2020·Journal of Chromatographic Science·Maimana A Magdy, Rehab M Abdelfatah
May 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·M MassaroG Lazzara
Dec 1, 2020·Journal of AOAC International·Ruth N Muchiri, Richard B van Breemen
Sep 3, 2020·Journal of Ethnopharmacology·Ilias MarmouziMourad Kharbach

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