PMID: 2161516May 25, 1990Paper

A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones

Nucleic Acids Research
J RileyA F Markham

Abstract

The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA fragments and terminal sequences are amplified using a vector specific primer and a linker specific primer. Sequence data derived from these terminal specific products can be used to design primers for a further round of screening to isolate overlapping clones. The method also provides a convenient method of generating Sequence Tagged Sites for the mapping of complex genomes.

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C N TraverR W Davis
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G A SilvermanS J Korsmeyer
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D L NelsonC T Caskey
Sep 29, 1989·Science·M OlsonD Botstein
Sep 8, 1988·Nature·A CoulsonY Kohara

❮ Previous
Next ❯

Citations

Jun 13, 1998·Genes, Chromosomes & Cancer·J AdélaïdeM J Pébusque
Oct 1, 1992·Molecular & General Genetics : MGG·A Kilian, A Kleinhofs
Dec 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J C MontgomeryL A Flaherty
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L Stubbs
Dec 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R D CoxH Lehrach
Jul 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H F SutherlandA M Frischauf
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·N C Casavant, S C Hardies
Jan 1, 1994·Somatic Cell and Molecular Genetics·J C FuscoeG Pilia
Jan 1, 1996·Immunogenetics·M T WalshA S Whitehead
Apr 1, 1995·Molecular Biotechnology·G RotmanY Shiloh
Apr 1, 1994·Molecular Biotechnology·M Ramsay
Aug 29, 2009·Applied Microbiology and Biotechnology·Yuki Tonooka, Masahiro Fujishima
Jun 28, 2007·Current Genetics·Virginie Ansanay GaleoteBruno Blondin
Jan 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M C PotierR Reeves
Feb 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·X J GuanS M Tilghman
Jul 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T P MoynihanP A Robinson
Oct 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S E AndersenA F Markham
Nov 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J HoyleE M Fisher
Jun 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·F RotomondoG F Carle
Apr 23, 2011·Molecular Genetics and Genomics : MGG·Cecilia Y ChanRobert H Schiestl
Aug 2, 2008·Extremophiles : Life Under Extreme Conditions·Damien MarsicJoseph D Ng
Jul 13, 2007·Marine Biotechnology·Laura SteindlerSven Beer
Mar 12, 2009·Molecular Biotechnology·Elena HilarioMark McNeilage
Sep 1, 1991·Trends in Genetics : TIG·C Wicking, B Williamson
Feb 1, 1994·Progress in Neurobiology·J W SentryK Kaiser
Sep 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D L Hartl, E R Lozovskaya
Mar 1, 1995·Genomics·J T RestonH C Riethman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.