A novel, rapid method to detect infectious HIV-1 from plasma of persons infected with HIV-1

Journal of Virological Methods
Alyssa CornallSharon R Lewin

Abstract

Efficient isolation of replication-competent virus from plasma of patients infected with HIV-1 is needed to characterize important clinical parameters of virus. However, addition of plasma to in vitro cultures results in clot formation. Blood from HIV-1 infected patients was collected in the presence of three commonly used anticoagulants (ACD, heparin and EDTA) and plasma was isolated. Plasma was then used to infect HIV-1 indicator cell lines (TZM-bl and GHOST) with spinoculation in the presence or absence of additional heparin and positively charged polymers. The presence of additional heparin during inoculation significantly reduced clot formation without affecting the sensitivity of HIV-1 infection in the GHOST cell line. However, heparin reduced the frequency of HIV-1 infection of the TZM-bl cell line. Using plasma from patients with HIV RNA>1000 copies/ml (n=58), the frequency of HIV-1 isolation was 92% in GHOST (n=51) and 54% in TZM-bl (n=26) cell lines. A sensitive method was developed for rapid isolation of infectious HIV-1 from plasma of patients with HIV RNA>1000 copies/ml that includes spinoculation and the addition of heparin during infection of GHOST cells. This technique could be used for rapid evaluation of viral...Continue Reading

References

Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M BabaE De Clercq
Dec 14, 1989·The New England Journal of Medicine·R W CoombsL Corey
Dec 14, 1989·The New England Journal of Medicine·D D HoM Alam
Jun 27, 1983·Annals of the New York Academy of Sciences·J J HardyJ McDonagh
Apr 1, 1994·Antimicrobial Agents and Chemotherapy·E De ClercqR Datema
Mar 23, 2000·The Journal of Clinical Investigation·G P RizzardiG Pantaleo
Sep 1, 1954·The Biochemical Journal·J W LYTTLETON
May 1, 1964·Journal of Clinical Pathology·S BRUCE
Jan 15, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Don K WalkerDennis A Smith
Nov 26, 2005·Journal of Virological Methods·Ayub Ali, Otto O Yang
Feb 18, 2009·The Journal of Antimicrobial Chemotherapy·Isabelle MaletAnne-Genevieve Marcelin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.